Recombinant Human Stromal-Cell Derived Factor-1 gamma/CXCL12γ (rHuSDF-1γ/CXCL12γ)

规格编号：C640030-0002
概述：CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. In recently study, Human CXCL12 is expressed as six isoforms that differ only in the Cterminal tail. And all SDF-1 isoforms undergo proteolytic processing of the first two Nterminal amino acids. Human SDF-1γ is synthesized as a 119 amino acid (a.a.) precursor that contains a 21 a.a. signal sequence and a 98 a.a. mature region and the SDF-1γ Cterminus binds heparin in secreted SDF-1γ, or targets the isoform to the nucleolus in the absence of a signal sequence. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for Tlymphocytes, monocytes, but not neutrophils. CXCL12 is a very important factor in carcinogenesis and the neovascularisation linked to tumor progression.
使用说明：This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Physical Appearance Sterile Filtered White lyophilized (freezedried) powder.
Purity Sterile Filtered White lyophilized (freezedried) powder
Source Escherichia coli.
Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 30100 ng/ml.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin Less than 0.1 EU/μg of rHuSDF-1γ/CXCL12γ as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
AA Sequence GKPVSLSYRC PCRFFESHVA RANVKHLKIL NTPNCALQIV ARLKNNNRQV CIDPKLKWIQ EYLEKALNKG RREEKVGKKE KIGKKKRQKK RKAAQKRKN