Stemgent® Purified anti-Mouse/Human Sox2 Antibody详细信息:
Product Overview
Stemgent™ Purified Rabbit anti-Mouse/Human Sox2 Antibody was screened on human and mouse embryonic stem (ES) cells using immunocytochemistry (ICC) and intracellular staining by flow cytometry (FC) and selected as the best Sox2 antibody available for researchers needing to demonstrate pluripotency. Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated ES cells and is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state1-3.
Stemgent® Purified anti-Mouse/Human Sox2 AntibodyProduct Specifications
Immunogen Synthetic human Sox2 peptide near the C-terminus (KLH-coupled)
Reactivity Mouse and human
Clone Polyclonal
Isotype Rabbit IgG
Formulation Phosphate-buffered solution pH 7.2 containing < 0.1% sodium azide, 0.5% BSA, 2 mM HEPES pH 7.5, 30 mM NaCl, 10% Glycerol.
Preparation The antibody was purified by protein A and peptide affinity chromatography.
Storage Store undiluted at 4oC and protected from light. Do not freeze.
Stability Stable for 6 months from date of receipt when stored as directed.
Quality Control Tested by flow cytometry and immunocytochemistry to ensure product quality.
Recommended Usage Dilute 1:100 for immunocytochemistry applications and 1:5 for flow cytometry applications. For general protocols for performing immunocytochemistry (ICC) and flow cytometry (FC) directly on ES cell lines, see Technical Documents on product page.
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