Stemgent® StainAlive™ DyLight™ 488 Mouse anti-SSEA-1 Antibody

Stemgent® StainAlive™ DyLight™ 488 Mouse anti-SSEA-1 Antibody详细信息：
Product Overview

Stemgent® StainAlive™ DyLight™ 488 Mouse anti-SSEA-1 Antibody was screened on live, unfixed mouse embryonic stem (ES) cells using immunocytochemistry (ICC). This antibody was selected as the best conjugated SSEA-1 antibody available for visualization of EC/ES/iPS cells, utilizing direct fluorescent staining and real-time imaging without fixation or killing the cells to direct visualization of the reprogramming process to iPS colonies. For use on live cells, this antibody has been specifically formulated with low endotoxin and no sodium azide and is confirmed to be free of bacteria, fungi, mycoplasma and mouse virus. The live ES cell morphology, proliferation, and expression of pluripotency markers did not change after staining with this antibody. The MC-480 antibody reacts with mouse and human stage-specific embryonic antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis X carbohydrate structure. It is expressed on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. It is widely used as a positive surface marker for mouse undifferentiated stem cells and a negative surface marker for human undifferentiated stem cells. Expression is down-regulated following differentiation of murine EC and ES cells, while the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression. SSEA-1 is associated with cell adhesion, migration and differentiation.

Stemgent® StainAlive™ DyLight™ 488 Mouse anti-SSEA-1 AntibodyProduct Specifications

Size / Quantity    50 µg (100 µl at 0.5 mg/ml)
Lot Number    1314
Immunogen    Mouse F9 Teratocarcinoma Stem Cells (X-irradiated)
Reactivity    Mouse
Clone    MC-480
Isotype    Mouse IgM, κ
Formulation    Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 1 EU/μg of the protein (< 0.1 ng/μg of the protein) as determined by the LAL assay. It is free from bacteria, fungi, mycoplasma, and mouse virus as tested.
Preparation    The antibody was purified by affinity chromatography, and conjugated with DyLight™ 488 under optimal conditions. The solution is free of unconjugated dye and free antibody.
Storage and Handling    Store at 4°C. Do not freeze. Light sensitive. This formulation contains no preservatives. Handle under aseptic conditions.
Stability    Stable for 6 months from date of receipt when stored as directed.
Quality Control    Tested by immunocytochemistry (Figure 1)
Recommended Usage    Dilute 1:100 for immunocytochemistry. For general protocols for performing viable cell immunocytochemistry directly on stem cells, see Technical Documents on the Stemgent® StainAlive™ DyLight™ 488 Mouse anti-SSEA-1 Antibody product page at www.stemgent.com/cat#09-0067.
Related Products    Stemgent® DyLight™ 488 anti-SSEA-1 Antibody (Cat. # 09-0070)
Stemgent® StainAlive™ DyLight™ 488 Mouse anti-Human TRA-1-81 Antibody (Cat. # 09-0069)
Notice to Purchaser    Purchaser represents and warrants that it will use Stemgent® StainAlive™ Dylight™ 488 Mouse anti-SSEA-1 Antibody purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Stemgent® StainAlive™ DyLight™ 488 Mouse anti-SSEA-1 Antibody
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