Stemgent® StainAlive™ DyLight™ 488 Mouse anti-Human TRA-1-60 Antibody

Stemgent® StainAlive™ DyLight™ 488 Mouse anti-Human TRA-1-60 Antibody详细信息：
Product Overview

The Stemgent® StainAlive™ DyLight™ 488 Mouse anti-Human TRA-1-60 Antibody was screened on live, unfixed human embryonic stem (ES) cells using immunocytochemistry (ICC). This antibody was selected as the best conjugated TRA-1-60 antibody available for visualization of ES/iPS (induced pluripotent stem) cells, utilizing direct fluorescent staining and real-time imaging without fixation or killing the cells. This antibody format is useful for direct visualization of the reprogramming process to iPS colonies. For use on live cells, this antibody has been specifically formulated with low endotoxin and no sodium azide and is confirmed to be free of bacteria, fungi, mouse virus and mycoplasma as tested. The live ES cell morphology, proliferation and expression of pluripotency markers did not change after staining with this antibody. Clone TRA-1-60 reacts with a pluripotent-stem-cell-specific antigen expressed on undifferentiated human embryonic stem (ES) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells. The expression of TRA-1-60 on human ES cells is down-regulated upon differentiation. The TRA-1-60 antibody recognizes a neuraminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein, which has been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.

Stemgent® StainAlive™ DyLight™ 488 Mouse anti-Human TRA-1-60 AntibodyProduct Specifications

Size / Quantity    50 µg (100ul at 0.5 mg/ml)
Immunogen    Human embryonal carcinoma (EC) cell line 2102Ep
Reactivity    Human
Clone    TRA-1-60
Isotype    Mouse IgM, κ
Formulation    Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 μm filter sterilized. Endotoxin level is < 1 EU/μg of the protein (< 0.1 ng/μg of the protein) as determined by the LAL test. It is free from bacteria, fungi, mouse virus and mycoplasma as tested.
Preparation    The antibody was purified by affinity chromatography, and conjugated with DyLight 488™ under optimal conditions. The solution is free of unconjugated dye and free antibody.
Storage    Store undiluted at 4°C. Do not freeze. Light sensitive. This StainAlive™ solution contains no preservative; handle under aseptic conditions.
Stability    Stable for 6 months from date of receipt when stored as directed.
Quality Control    Tested by immunocytochemistry (ICC) to ensure product quality (Fig. 1).
Recommended Usage    Use antibody at 5 µg/ml (1:100) in ICC. For general protocols for performing live immunocytochemistry ES cell lines, visit www.stemgent.com/cat#09-0068.
Related Products    
Stemgent® StainAlive™ DyLight™ 488 Mouse IgM, κ Isotype Control Antibody (Cat. # 09-0072)
Notice to Purchaser    Purchaser represents and warrants that it will use Stemgent® StainAlive™ DyLight™ 488 Mouse anti-Human TRA-1-60 Antibody purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Trademark    DyLight™ is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Stemgent® StainAlive™ DyLight™ 488 Mouse anti-Human TRA-1-60 Antibody
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