PRODUCT SOURCE
Recombinant Human Activin A /INHBA Protein,HEK293 expressed,ActiveMax (rh Activin A) Gly 311 - Ser 426 (Accession # AAH07858.1) was produced in human 293 cells (HEK293) at ACROBiosystems.
MOLECULAR CHARACTERIZATION
rh ACVR2A / ACVR2 Fc Chimera is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 40 kDa. The predicted N-terminus is Ala 20. DTT-reduced Protein migrates as 30-33 kDa in SDS-PAGE due to different glycosylation.
BIOLOGICAL ACTIVITY
The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse MPC-11 cells. <1 ng/mL, corresponding to a specific activity of >1000000 Unit/mg.
PURITY
>95% as determined by SDS-PAGE.
ENDOTOXIN
Less than 1.0 EU per μg of the rh Activin A by the LAL method.