Processing Time | 18 min |
DNA Purity | Eluted DNA is ultrapure and well suited for transfection, sequencing, cloning, plasmid mediated gene silencing, in vitro transcription, and other sensitive applications. |
Plasmid DNA Size | Up to 25 kb |
Plasmid DNA Yield | Up to 300 µg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized) |
Recovery Volume | ≥ 100 µl of ZymoPURE™ Elution Buffer or DNase free water |
Equipment Needed | Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge. |
Recommended Sample Volume | 50 ml E. coli Culture. |