描述SYBR® Select Master Mix is a superior and cost-effective solution for your real-time PCR applications.
• Specific—minimize primer-dimer and non-specific amplification • Reproducible and sensitive—consistent amplification across a wide dynamic range • Bright—contains SYBR® GreenER™ dye for maximum brightness • Carry-over contamination control—contains heat-labile UDG • Broad instrument compatibility
Formulated for Maximum Specificity SYBR® Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq® DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.
Obtain Reproducible Results Across a Wide Dynamic Range SYBR® Select Master Mix is specially formulated to provide robust results from 100 ng to 0.1 pg cDNA per reaction and can reliably detect a single copy of genomic DNA.
Contains SYBR® GreenER™ Dye and Heat-labile UDG • SYBR® GreenER™ dye is less inhibitory to PCR than SYBR® Green I dye resulting in brighter signals • Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control
Instrument Compatibility SYBR® Select Master Mix is used in standard cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments. It is also compatible with the Bio-Rad IQ™5, Roche LightCycler® LC480, and Stratagene® MX3005P® systems.
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
内容和储存
The 2x mix contains SYBR® GreenER™ dye, AmpliTaq® DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 5 mL tube, sufficient for 500, 20 µL reactions (10 µL Master Mix per reaction) . Store at 2 - 8 °C.详细说明
常用规格
Detection Method:
SYBR
Polymerase:
AmpliTaq® DNA Polymerase
Purity or Quality Grade:
UP (Ultra Pure)
Platform:
StepOne™, Standard Mode, ViiA™ 7 System, 7900HT System, Standard Mode, StepOnePlus™, Standard Mode, 7500 System, QuantStudio™ 12K Flex
Reaction Speed:
Standard
Sample Type (General):
DNA
Sample Type (Specific):
cDNA, Genomic DNA
Primer-Probe Compatibility:
Unlabeled Oligos
Form:
Liquid
Format:
Vial(s)
Hot Start:
Built-In Hot Start
PCR Method:
qPCR
Label or Dye:
SYBR® GreenER™
Product Size:
500 reactions
cGMP Compliance:
non-cGMP Compliant
Shipping Condition:
Wet Ice
Regulatory Statement:
For Research Use Only. Not for use in diagnostic procedures.