Anti-Melanoma HMB45/Melanoma/Melan-A/MART-1黑色素瘤相关抗原/黑色素-A抗体

本公司提供科研Melanoma HMB45/Melanoma/Melan-A/MART-1黑色素瘤相关抗原/黑色素-A抗体，抗体质量可靠，订购Melanoma HMB45/Melanoma/Melan-A/MART-1黑色素瘤相关抗原/黑色素-A抗体请联系在线客服或者销售人员。
抗体参数如下>>>>
中文名称：黑色素瘤相关抗原/黑色素-A抗体
英文名称：Anti-Melanoma HMB45/Melanoma/Melan-A/MART-1
货号：bs-0051R
抗体来源：兔
克隆类型：多克隆
蛋白分子量：predicted molecular weight: 13kDa
纯化方法：affinity purified by Protein A
交叉反应：hu
测试应用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
产品背景介绍：Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre existing benign nevus, which occurs most often in the skin but also may involve other sites.Function : Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.Subunit : Interacts with PMEL. Interacts with GPR143.Subcellular Location : Endoplasmic reticulum membrane; Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome. Note=Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.Tissue Specificity : Expression is restricted to melanoma and melanocyte cell lines and retina.信号中间体（Signaling Intermediates） MelanA/MART-1是分化肿瘤抗原类别中具有代表性的成员，它表达于正常的黑素细胞和大多数原发及转移的黑色素瘤中，故称：黑色素瘤相关抗原。产品图片Tissue/cell: mouse melanoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Melanoma HMB45/Melanoma/Melan-A/MART-1 Polyclonal Antibody, Unconjugated(bs-0051R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining