CRL-1592 IEC-6 大鼠小肠隐窝上皮细胞_详细资料

产品信息

数量：大量生长状态：正常年限： 2020 运输方式：常温器官来源：大鼠是否是肿瘤细胞：否免疫类型：查询相关资料物种来源：大鼠相关疾病：查询相关资料组织来源：大鼠小肠品系：查询相关资料 ATCC Number： CRL-1592 供应商： ATCC

CRL-1592 IEC-6 大鼠小肠隐窝上皮细胞，原代细胞|细胞系|细胞株|菌种；细胞库管理规范，提供的细胞株背景清楚，提供参考文献和培养条件！

CRL-1592 IEC-6 大鼠小肠隐窝上皮细胞的详细介绍

CRL-1592 IEC-6 大鼠小肠隐窝上皮细胞

ATCC^® Number: CRL-1592™ Price:

Designations: IEC-6

Depositors: A Quaroni

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus (rat)

Morphology: epithelial

Source: Organ: small intestine
Tissue: epithelium
Disease: normal

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host (Roche FuGENE® Transfection Reagents)

Gender: male

Comments: Cell growth is inhibited by cortisol.
The cells express antigens characteristic of intestinal epithelia. [22491]
The depositor notes that the cells maintain a constant growth rate and cell morphology until approximately the 20th passage, thereafter, either cell growth rapidly declines or morphological changes are observed.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 Unit/ml bovine insulin, 90%; fetal bovine serum, 10%
Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Twice per week

Preservation: Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase

您可能感兴趣的产品