T3 Total ELISA 甲状腺素共有ELISA检测试剂盒

产品描述

Category Name: Thyroid Markers

Method: ELISA

Principle: ELISA

Controls Standards: 0,0.5,1.0,2.5,5.0,7.5 ng/ml

Sensitivity: 0.04ng/ml

Sample: 50 µl

Runtime： 75 min

Wavelength： 450 nm

Safety： Please refer to MSDS

Shelflife： 18 Months

INTENDED USE

The Calbiotech Triiodothyronine (T3) ELISA Kit is intended for the quantitative measurement of Triiodothyronine (T3) in human serum or plasma.

Summary and explanation

Triiodothyronine (T3) is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T3 is decreased in hypothyroid patients and is increased in hyperthyroid patients, graves disease and pregnancy.

PRINCIPLE OF THE TEST

The CBI T3 is a solid phases competitive ELISA. The samples, assay buffer and T3 enzyme conjugate are added to the wells coated with anti-T3 monoclonal antibody. T3 in the patient’s serum competes with a T3 enzyme conjugate for binding sites. Unbound T3 and T3 enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T3 in the samples. A standard curve is prepared relating color intensity to the concentration of the T3.