增加ACRIFLAVINE至终浓度10mg/升
Vial contents (each vial is sufficient for 2,250mls of medium) | per vial | per litre |
Nalidixic acid | 90.0mg | 40.0mg |
Cycloheximide | 112.5mg | 50.0mg |
Acriflavine hydrochloride | 22.5mg | 10.0mg |
Directions
Aseptically add 10ml of sterile distilled water to one vial and invert gently to dissolve. Aseptically add the vial contents to 2.25 litres of Listeria Enrichment Broth Base, cooled to 50°C.