Stemgent® Purified Mouse anti-Human CD45 Antibody

Stemgent® Purified Mouse anti-Human CD45 Antibody was screened on normal human mesenchymal stem cells (MSCs) by immunocytochemistry (ICC) and flow cytometry (FC) and selected as the best CD45 negative selection antibody for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification^1-3. CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation^4-5.

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2. Dvorakova, J., Hruba, A., Velebny, V., Kubala, L. (2008) Isolation and characterization of mesenchymal stem cell population entrapped in bone marrow collection sets. Cell Biol Int 32: 1116-25.
3. Anker, P.S., Noort, W.A., Scherjon, S.A., Kleijburg-van der Keur, C., Kruisselbrink, A.B., van Bezooijen, R.L., Beekhuizen, W., Willemze, R., Kanhai, H.H., Fibbe, W.E. (2003) Mesenchymal stem cells in human second-trimester bone marrow, liver, lung, and spleen exhibit a similar immunophenotype but a heterogeneous multilineage differentiation potential. Haematologica 88: 845-52.
4. Thomas, M.L. (1989) The Leukocyte Common Antigen Family. Ann. Rev. Immunol. 7: 339-69.
5. Clark, E.A., Ledbetter J.A. (1989) Leukocyte cell surface enzymology: CD45 (LCA, T200) is a protein tyrosine phosphatase. Immunol Today. 10(7): 225-8.