Stemgent® Purified Mouse anti-Human CD34 Antibody

Stemgent® Purified Mouse anti-Human CD34 Antibody was screened on normal human mesenchymal stem cells (MSCs) by immunocytochemistry (ICC) and flow cytometry (FC) and selected as the best CD34 negative selection antibody for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification^1-3. CD34 is a surface glycophosphoprotein expressed on developmentally early lymphohematopoietic stem and progenitor cells with a molecular weight of about 105-120 kD. It is selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands^4-5.

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2. Dvorakova, J., Hruba, A., Velebny, V., Kubala, L. (2008) Isolation and characterization of mesenchymal stem cell population entrapped in bone marrow collection sets. Cell Biol Int 32: 1116-25.
3. Anker, P.S., Noort, W.A., Scherjon, S.A., Kleijburg-van der Keur, C., Kruisselbrink, A.B., van Bezooijen, R.L., Beekhuizen, W., Willemze, R., Kanhai, H.H., Fibbe, W.E. (2003) Mesenchymal stem cells in human second-trimester bone marrow, liver, lung, and spleen exhibit a similar immunophenotype but a heterogeneous multilineage differentiation potential. Haematologica 88: 845-52.
4. Brown, J., Greaves, M.F., Molgaard H.V. (1991) The gene encoding the stem cell antigen, CD34, is conserved in mouse and expressed in haemopoietic progenitor cell lines, brain, and embryonic fibroblasts. Int Immunol. 3(2): 175-84.
5. Krause, D.S., Fackler, M.J., Civin, C.I., Stratford May, W. (1996) CD34: Structure, Biology, and Clinical Utility. Blood (87)1: 1-13.