Stemgent® Purified Mouse anti-Human CD166 Antibody

Stemgent® Purified Mouse anti-Human CD166 Antibody was screened on normal human mesenchymal stem cells (MSC) by flow cytometry (FC) and selected as the best CD166 antibody for characterizing and/or isolating human mesenchymal stem cells. CD166, also known as ALCAM (activated leukocyte cell adhesion molecule), is a member of the immunoglobulin superfamily with five extracellular immunoglobuli -like domains. It facilitates heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. While expressed in a wide variety of tissues and cells, ALCAM is restricted to subsets of cells usually involved in dynamic growth and/or migration processes^1-2. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources^3-5.

1. van Kempen, L.C., Nelissen, J.M., Degen, W.G., Torensma, R., Weidle, U.H., Bloemers, H.P., Figdor, C.G., Swart, G.W. (2001) Molecular basis for the homophilic activated leukocyte cell adhesion molecule (ALCAM)-ALCAM interaction. J Biol Chem 276: 25783-90.
2. Bowen, M.A., Patel, D.D., Li, X., Modrell, B., Malacko, A.R., Wang, W.C., Marquardt, H., Neubauer, M., Pesando, J.M., Francke, U., et al. (1995) Cloning, mapping, and characterization of activated leukocyte-cell adhesion molecule (ALCAM), a CD6 ligand. J Exp Med 181: 2213-20.
3. Dvorakova, J., Hruba, A., Velebny, V., Kubala, L. (2008) Isolation and characterization of mesenchymal stem cell population entrapped in bone marrow collection sets. Cell Biol Int 32: 1116-25.
4. Lian, Q., Lye, E., Suan Yeo, K., Khia Way Tan, E., Salto-Tellez, M., Liu, T.M., Palanisamy, N., El Oakley, R.M., Lee, E.H., Lim, B., Lim, S.K. (2007) Derivation of clinically compliant MSCs from CD105+, CD24- differentiated human ESCs. Stem Cells 25: 425-36.
5. Anker, P.S., Noort, W.A., Scherjon, S.A., Kleijburg-van der Keur, C., Kruisselbrink, A.B., van Bezooijen, R.L., Beekhuizen, W., Willemze, R., Kanhai, H.H., Fibbe, W.E. (2003) Mesenchymal stem cells in human second-trimester bone marrow, liver, lung, and spleen exhibit a similar immunophenotype but a heterogeneous multilineage differentiation potential. Haematologica 88: 845-52.