Stemgent® Purified Mouse anti-Human CD105 Antibody

Stemgent® Purified Mouse anti-Human CD105 Antibody was screened on normal human mesenchymal stem cells (MSC) by immunocytochemistry (ICC) and flow cytometry (FC) and selected as the best CD105 antibody for characterizing and/or isolating human mesenchymal stem cells. CD105 (also known as Endoglin) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 95 kDa. It is mostly present on cellular lineages within the vascular system and connective tissues¹. CD105 has been identified as a highly expressed antigen on human bone marrow, embryonic stem cells, and other source-derived mesenchymal stem cells (MSC)^2-5.

1. Fonsatti, E., Del Vecchio, L., Altomonte, M., Sigalotti, L., Nicotra, M.R., Coral, S., Natali, P.G., Michele, M. (2001) Endoglin: An Accessory Component of the TGF-b-Binding Receptor-Complex With Diagnostic, Prognostic, and Bioimmunotherapeutic Potential in Human Malignancies. Journal of Cellular Physiology 188: 1-7.
2. Barry, F.P., Boynton, R.E., Haynesworth, S., Murphy, J.M., Zaia, J. (1999) The Monoclonal Antibody SH-2, Raised against Human Mesenchymal Stem Cells, Recognizes an Epitope on Endoglin (CD105). Biochemical and Biophysical Research Communications 265: 134-139.
3. Campagnoli, C., Roberts, I.A., Kumar, S., Bennett, P.R., Bellantuono, I., Fisk, N.M. (2001) Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow. Blood 98: 2396-402.
4. Lee, R.H., Kim, B., Choi, I., Kim, H., Choi, H.S., Suh, K., Bae, Y.C., Jung, J.S. (2004) Characterization and expression analysis of mesenchymal stem cells from human bone marrow and adipose tissue. Cell Physiol Biochem 14: 311-24.
5. Lian, Q., Lye, E., Yeo, K.S., Way Tan, E.K., Salto-Tellez, M., Liu, T.M., Palanisamy, N., El Oakley, R.M., Lee, E.H., Lim, B., Limb, S.K. (2007) Derivation of Clinically Compliant MSCs from CD105+, CD24- Differentiated Human ESCs. Stem Cells 25: 425-436.