Caspase-8 1007-1 兔单克隆抗原抗体_详细资料

产品信息

抗体英文名： Caspase-8 是否单克隆：单克隆数量：大量适应物种： human,rat 应用范围： wb 抗原来源： rabbit 宿主： rabbit

Description

Caspase-8 (MACH/FLICE/Mch5) is a member of a family of cysteine-requiring aspartate proteases that participate in the intracellular signaling cascade leading to apoptosis, or programmed cell death (1-4). Caspase-8 is recruited to death-inducing signaling complex (DISC) CD95 (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) and is subsequently activated by proteolytic cleavage into two residues, p10 and p18 (1-4). Active caspase-8, the first in the caspase signaling cascade, signals downstream caspases to eventually induce apoptosis.

Recommended Applications

WB, FC, IP

Applications and Recommended Dilution Factors

WB IHC ICC FC IP

1:500 1:20 1:40

Species Reactivity*

Human Mouse Rat

Positive Negative Positive *Cross reactivity determined by western blot only.

Product Data

A. Western blot analysis on HeLa cell lysate using anti-Caspase-8 RabMAb (catalog #1007-1), 1:500 dilution.

Specificity

A synthetic peptide corresponding to N-terminal residues of human Caspase-8 subunit p18 was used as immunogen. The antibody should recognize both pro-form (55kDa) and p18 cleaved-form of Caspase-8.

Storage Buffer & Conditions

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Alternative Names

CASP8, MCH5, Caspase-8 subunit p10, Apoptotic cysteine protease;Apoptotic protease Mch-5;CAP4

Description References

1. Muzio, M. et al. Cell 85: 817-827 (1996)
2. Boldin, M.P. et al. Cell 85: 803-815 (1996)
3. Srinivasula, S.M. et al. Proc. Natl. Acad. Sci. U S A 93(25): 14486-91 (1996)
4. Medema, J.P. et al. EMBO J. 16(10): 2794-804 (1997)

Product References

1. Kaneko M, Kaneko S, Suzuki K., Prolonged low-dose administration of the cyclooxygenase-2 inhibitor celecoxib enhances the antitumor activity of irinotecan against neuroblastoma xenografts ,Cancer Sci. 100 (2009) 2193-2201
[Application: WB]

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