DBCO-amine,1255942-06-3, 二苯基环辛炔-氨基

中文名称：氮杂二苯并环辛炔胺，二苯基环辛炔-氨基

中文别名：氨基丙酰氮杂二苯并环辛炔

英文名称：Azadibenzocylooctyne-amine

英文别名：Dibenzocyclooctyne-amine;DBCO-NH2;DBCO-amine;ADIBO-AMINE;

CAS号：1255942-06-3

分子式：C18H16N2O

分子量：276.33200

精确质量：276.12600

PSA：46.33000

LogP：3.04710

结构式：

名称：DBCO-Amine   MW:276.33   CAS:1255942-06-3

英文简介：

A carboxyl-reactive building block containing DBCO moiety and a terminal primary amine group.

DBCO-Amine is a simple DBCO-containing reagent possessing a terminal primary amines group (–NH2) that reacts with carboxyl groups in the presence of activators (e.g. EDC, or DCC) or with activated esters (e.g. NHS esters) forming stable amide bonds. A short spacer arm adds minimal mass to modified molecules (273.3 daltons).

名称：DBCO-PEG4-Amine   MW:523.62

英文简介：

A carbonyl-reactive building block with enhanced solubility in aqueous media.

DBCO-PEG4-Amine is a polyethylene glycol-containing reagent with terminal primary amines (–NH2) that reacts with carboxyl groups in the presence of activators (e.g. EDC, or DCC) or with activated esters (e.g. NHS esters) forming stable amide bonds. A hydrophilic spacer arm enhances solubility in aqueous buffers.

名称：DBCO-NHS Ester   MW：402.40

英文介绍：

DBCO-NHS Ester reacts with primary amines

This product is not recommended for labeling of proteins or any other biopolymers in aqueous buffers due to poor aqueous solubility.

名称: DBCO-C6-NHS Ester   MW:430.45

英文介绍：

An activated ester with extended 6-carbon atom long spacer.

DBCO-NHS Ester reacts with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form covalent bonds. Extended 6-carbon spacer arm is beneficial for some application. This reagent has limited shelf life; however, corresponding acid and its derivatives are found to be stable over long period of time. DBCO-Sulfo-NHS-Ester, a water-soluble version of this product is also available for labeling experiments where the presence of organic solvents is highly undesirable.

名称：DBCO-PEG5-NHS Ester  MW:693.74

英文简介：

An amine-reactive labeling reagent with enhanced water stability and solubility.

DBCO-PEG5-NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a covalent bond. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.

名称：DBCO-PEG4-NHS Ester  MW:649.68

英文简介：

An amine-reactive labeling reagent with enhanced water solubility provided by a PEG4 spacer.

DBCO-PEG4-NHS Ester reacts specifically and efficiently with a primary amine (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form a covalent bond. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.

名称：DBCO-Acid   MW:305.11

英文简介：

DBCO-Acid is an amine reactive, non-activated cyclooctyne building block.

The use of strained cyclooctynes instead of terminal alkynes allows for overcoming a major drawback of copper-catalyzed azide-alkyne cycloaddition (CuAAC) - the need for the toxic copper catalyst. The ring strain in cyclooctynes, such as DBCO-Acid makes them highly reactive in the cycloaddition reaction. This reaction was termed as the strain-promoted alkyne-azide cycloaddition (SPAAC) or copper-free click reaction and has been widely used for bioconjugation and for labeling living cells and living animals.

名称：DBCO-PEG5-Acid    MW:596.67

英文简介：

An amine-reactive building block with enhanced stability and solubility in aqueous media.

DBCO-PEG5-Acid is a polyethylene glycol-containing reagent with terminal carboxylic acid (–COOH) that can be reacted with primary or secondary amine groups in the presence activators (e.g. EDC) forming a stable amide bond. The PEG spacer arm is hydrophilic and provides greater solubility to labeled molecules compared to reagents having only hydrocarbon spacers. The PEG spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance involved with reacting with complementary azido-modified molecules.

名称：DBCO-PEG4-Acid  MW:552.62

英文简介：

An amine-reactive building block with enhanced solubility in aqueous media.

DBCO-PEG4-Acid is a polyethylene glycol-containing reagent with terminal carboxylic acid (–COOH) that can be reacted with primary or secondary amine groups in the presence activators (e.g. EDC) forming a stable amide bond. The PEG spacer arm is hydrophilic and provides greater solubility to labeled molecules compared to reagents having only hydrocarbon spacers

名称：DBCO-PEG4-4-Formylbenzoate    MW:655.77

英文简介：

A heterobifunctional reagent for labeling aminooxy-containing compounds.

DBCO-PEG4-4-Formylbenzoate reacts specifically and efficiently with an aminooxy group at pH 4-5 to form a covalent oxime bond. The oxime ligation with aromatic aldehydes is highly chemoselective and is compatible with other functional groups present in peptides or biomolecules. An aniline catalysis can be used to activate aromatic aldehyde towards an aminooxy group and greatly enhance the rate of ligation reaction.

名称：ThioLinker-DBCO

英文简介：

A unique, water-soluble, bis-alkylating reagent that allows for site-specific labeling of antibodies under mild aqueous conditions.

The traditional sulfhydryl labeling chemistry cleaves disulfide bridges and leaves them in “open form”, which may compromise the stability and function of an antibody or other proteins. ThioLinkerTM-DCBO re-bridges the disulfide bond, and enhances the structural stability of the protein. In addition, conjugates prepared with ThioLinkerTM reagents benefits from reduced heterogeneity and better stability.

名称：DBCO-Maleimide  MW:427.75

英文简介：

A short-length, sulfhydryl-reactive labeling reagent that contains a short hydrocarbon spacer.

DBCO-Maleimide enables simple and efficient incorporation of DBCO moiety onto cysteine-containing peptides or other thiol-containing molecules. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bonds. A short spacer arm adds minimal mass to modified molecules (427.4 daltons).

名称：DBCO-PEG4-Maleimide  MW:674.74

英文简介：

A mid-length, sulfhydryl-reactive labeling reagent with enhanced water solubility provided by a PEG4 spacer.

DBCO-PEG4-Maleimide enables simple and efficient incorporation of DBCO moiety onto antibodies, cysteine-containing peptides and other thiol-containing molecules. The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in the solution. The PEG spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary azide-containing molecules.

名称：Bis-sulfone-PEG4-DBCO   MW：1006.19

英文简介：

A bis-alkylating labeling reagent used to create conjugates with enhanced structural stability.

Bis-sulfone-PEG4-DBCO enables site-specific incorporation of DBCO moiety onto antibodies to form well defined conjugates. Unlike conjugates prepared through conventional, maleimide-based labeling chemistry that leaves disulfide bridges in “open form” and produces a heterogeneous mixture labeled species with DAR 1-8, conjugates prepared with bis-sulfone reagents benefits from reduced heterogeneity and enhanced stability.

名称：Bis-sulfone-PEG3-Azide  MW:700.82

英文简介：

A bis-alkylating labeling reagent used to create well-defined conjugates with enhanced structural stability.

Bis-sulfone-PEG3-Azide enables site-specific incorporation of an azide group onto antibodies to form well defined conjugates. Unlike conjugates prepared through conventional, maleimide-based labeling chemistry that leaves disulfide bridges in “open form” and produces a heterogeneous mixture labeled species with DAR 1-8, conjugates prepared with bis-sulfone reagents benefits from reduced heterogeneity and enhanced stability.

DBCO Reagents-Bifunctional Crosslinkers产品详细英文介绍和使用说明：

名称：DBCO-PEG4-DBCO   MW:854.92

英文简介：

An azide-to-azide homobifunctional cross-linker with a hydrophilic PEG4 spacer arm.

DBCO-PEG4-DBCO reacts specifically and efficiently with an azide group (e.g., a side chain of L-azidohomoalanine) to form a stable triazole moiety.

名称：TCO-PEG12-DBCO

英文简介：

An azide-to-tetrazine heterobifunctional cross-linker with a hydrophilic PEG12 spacer arm.

Heterobifunctional reagents are used for converting azido-containing biomolecules into TCO-modified biomolecules. TCO-PEG12-DBCO can serve as a heterobifunctional azide-to-tetrazine cross-linker for covalently linking azido- and tetrazine-modified peptides or biopolymers. The PEG12 spacer arm provides a long and flexible connection that minimizes steric hindrance involved with ligation to complementary reagent.

名称：Methyltetrazine-DBCO

英文简介：

A water-soluble azide-to-TCO heterobifunctional crosslinker.

Heterobifunctional reagents used for converting azido-containing peptides or protein into tetrazine-modified peptides or protein without the need for catalyst or axillary reagents. Tetrazine-DBCO can serve as a heterobifunctional azide-to-TCO cross-linker for covalently linking azido- and TCO-modified peptides or biopolymers.

名称：Tetrazine-PEG12-DBCO

英文简介：

An azide-to-TCO heterobifunctional crosslinker with a hydrophilic PEG12 spacer arm.

Heterobifunctional reagents used for converting azido-containing peptides or protein into tetrazine-modified peptides or protein without the need for catalyst or axillary reagents. Tetrazine-PEG12-DBCO can serve as a heterobifunctional azide-to-TCO cross-linker for covalently linking azido- and TCO-modified peptides or biopolymers.

名称：DBCO-PEG4-4-Formylbenzoate

英文简介：

A heterobifunctional reagent for labeling aminooxy-containing compounds.

DBCO-PEG4-4-Formylbenzoate reacts specifically and efficiently with an aminooxy group at pH 4-5 to form a covalent oxime bond. The oxime ligation with aromatic aldehydes is highly chemoselective and is compatible with other functional groups present in peptides or biomolecules. An aniline catalysis can be used to activate aromatic aldehyde towards an aminooxy group and greatly enhance the rate of ligation reaction.

名称：WS DBCO-Biotin

英文简介：

A water-soluble, azide-reactive biotinylation reagent.

DBCO reacts with an azide to produce a stable triazole, also referred to as the Cu(I)‐free or strain-promoted click reaction. The azide group, which is the reactive target of DBCO-activated biotin, can be introduced into proteins or other cellular targets via azide labeling reagents or through in vivo labeling with azide-derivatives of naturally occurring metabolic building blocks. This reaction  extremely selective and biocompatible, such that the complimentary reagents can form a covalent bond within richly functionalized biological systems, and, in some cases, living organisms.

名称：photoclevable DBCO-Biotin

英文简介：

An azide-reactive, photoclevable biotin probe that allows for reagent-free release of the captured biomolecules from streptavidin.Extraordinary strength of the streptavidin-biotin interaction allows for efficient capturing of even highly dilute targets; however, it makes recovery of proteins from affinity resins challenging.

名称：DBCO-PEG4-Desthiobiotin

英文简介：

An azide-reactive desthiobiotin reagent that allows easy elution from streptavidin.

Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.

名称：WS-PC DBCO-Biotin

英文简介：

A water-soluble, photoclevable biotin probe that allows for reagent-free release of the captured biomolecules from streptavidin.

Extraordinary strength of the streptavidin-biotin interaction allows for efficient capturing of even highly dilute targets; however, it makes recovery of proteins from affinity resins challenging. Photocleavable biotin probes eliminate a major limitation of the streptavidin-biotin affinity purification. This reagent contains a biotin moiety linked to an azide moiety through a spacer arm containing a photocleavable moiety. Captured biomolecules can be efficiently photoreleased, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2).

名称：Dde Biotin-DBCO

英文简介：

A hydrazine cleavable biotinylation reagent for selective enrichment of azide-tagged biopolymers via SPAAC reaction.

Extraordinary strength of the streptavidin-biotin interaction allows for efficient capturing of even highly dilute targets; however, it makes recovery of proteins from affinity resins challenging. Dde Biotin-DBCO probe eliminates a major limitation of the streptavidin-biotin affinity purification. This reagent contains a biotin moiety linked to an alkyne moiety through a spacer arm containing a Dde linker. Captured biomolecules can be efficiently released, typically >90%.

名称：DBCO-S-S-PEG3-Biotin

英文简介：

A cleavable biotinylation reagent for specific enrichment azide-tagged biomolecules.

DBCO-S-S-PEG3-Biotin enables simple and efficient biotin labeling of azide-tagged antibodies, proteins and other macromolecules. The DBCO group reacts with an azide to produce an stable triazole, which is also referred to as the Cu(I)-free or strain-promoted click reaction. The hydrophilic PEG spacer arm imparts water solubility and reduces aggregation of labeled proteins stored in the solution. The integral disulfide bond enables the biotin group to be cleaved from the labeled molecule using DTT or other reducing agents.