培养基和添加剂 Complete Growth Medium and Culture Conditions | RPMI-1640(GIBCO,货号31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L),90%;胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。 |
REFERENCE | Chinese Obstetrics Gynecology 29:162-164,1994 The Practical Journal of Cancer 18(3):266-269,2003 Chin J Cell Med Immunol 19(1):41-44,2003 Shanghai Immunol Journal 23(1):164-167,2003 |