North2South Biotin Random Prime DNA Labeling Kit 生物素标记DNA试剂盒_详细资料

产品信息

英文名： North2South Biotin Random Prime DNA Labeling Kit 供应商： qcbio 数量：大量规格： 10rxn kit

The North2South Random Prime Labeling Kit is based on the procedure of Feinberg and Vogelstein(Ref.4,5). Random heptanucleotides containing all possible sequences are annealed to a denatured DNA template. These act as primers for complementary strand synthesis by DNA Polymerase (Klenow fragment, 3'-5' exo-). Biotinylated dNTPs in the reaction mix are incorporated into the newly synthesized DNA. This protocol yields biotin-labeled DNA of high activity for use as probes in hybridization experiments such as Southern and Northern hybridizations.

Sufficient reagents for 10 reactions.**

Kit contains:

5X Heptanucleotide Mix 100 µl

5X Deoxynucleotide Mix 80 µl

10X Reaction Buffer 50 µl

Klenow DNA Polymerase 10 µl

Non-biotinylated control DNA 5 µl

5M Ammonium Acetate 1 ml

0.5M EDTA 1 ml

Nuclease-free Water 1 ml

Biotin-11-dUTP 20 µl

*Each reaction yields probe sufficient for approximately three 10 x 10 cm blots.
"The Control DNA in this kit is covered under U.S. Patent No. 4,766,072. This Control DNA is intended for the research use described within the technical literature of the accompanying kit. No other use is authorized. Please contact Promega Corporation for applications relating to all such other use."
温馨提示：不可用于临床ZL。

您可能感兴趣的产品

North2South Biotin Random Prime DNA Labeling Kit 生物素标记DNA试剂盒

产品信息

联系我们

关注我们

Sufficient reagents for 10 reactions.**
Kit contains:
5X Heptanucleotide Mix	100 µl
5X Deoxynucleotide Mix	80 µl
10X Reaction Buffer	50 µl
Klenow DNA Polymerase	10 µl
Non-biotinylated control DNA	5 µl
5M Ammonium Acetate	1 ml
0.5M EDTA	1 ml
Nuclease-free Water	1 ml
Biotin-11-dUTP	20 µl