关于 Biotin Antibody Agarose 的详细介绍
| Product Description | The biotin antibody is developed using biotin-KLH conjugates and purified with biotin-agarose affinity chromatography. The purified biotin antibody was chemically linked to beaded agarose through a stable covalent linkage. The immobilized biotin antibody on beaded agarose could be utilized for reversible immunoprecipitation and affinity purification of both biotinylated antibodies and biotinylated proteins. |
| Formulation | 1 mL beads in 2 mL slurry with glycerol |
| Antibody Immobilized | 10 mg of biotin antibody/mL agarose |
| Binding Capacity | > 1 mg/mL of biotinylated IgY |
| Applications | IP; Immuno-affinity chromatography; 10 mg/mL for proteomic study |
| Scientific Description | Biotin is a vitamin, which binds strongly and irreversibly to avidin. Biotin is commonly used for labelling reagents. Anti-biotin antibody binds to biotin strongly but reversibly, the binding could be competitively eluted with free biotin. |
| Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Do not aliquot and shake throughly before use. Expiration date is one year from date of shipping if properly stored. |
蛋白质翻译后生物素化修饰是新的PTM研究领域，Nature刊登的科技文献指出： 通过比较Sigma,SantaCruz的同类产品，只有ImmuneChem的抗生物素抗体Agarose填料(货号：ICP0615）达到PTM蛋白质组学质谱分析应用的质量。
抗生物素抗体Agarose填料(货号：ICP0615）也是非破坏性（Non-invasive)纯化Biotin-tag重组蛋白的重要填料,可不必用蛋白变性溶液而直接用游离生物素洗脱。 比传统的Avidin， Streptavidin Agarose要更理想，更可靠！
根据哈佛大学发表的文章数据显示，用我们的产品进行抗生物素蛋白富集和质谱分析，在数百种蛋白质上产生了1,600个以上的生物素化位点，所能检测到的生物素化位点比Sigma,SantaCruz的同类产品能多30倍！
文章链接：https://www.nature.com/articles/nmeth.4465#auth-1
| ImmuneChem Biotin Antibody Agarose produced the best results in research article published in: Nature Methods
Volume 14, Issue 12, 16 October 2017, Pages 1167–1170 |
| The binding affinity of anti-biotin antibodies could improve the elution of biotinylated peptides. This table shows the difference in the # of unique peptides when samples are biotinylated. | Biotin antibody compared to non-biotin samples |
| Immunechem’s Biotin Antibody Agarose (ICP0615) yielded the highest number of biotinylated peptides compared to Sigma-Aldrich and Santa Cruz. For more details, please refer to:
Nature Methods Volume 14, Issue 12, 16 October 2017, Pages 1167–1170 |
ImmuneChem（ZG）
南宁市奥思特科技有限责任公司 萧廉明
地址：南宁市高新区科园大道68号东盟慧谷11栋203-205室
电话：18775373503 QQ：2602622833
公司网站：http://www.ost-biotech.com/
技术支持：（Hao Xiao博士）immunechem@yahoo.com
我们可以为您提供下列产品和服务：
1：修饰蛋白/多肽蛋白质组学研究所用的抗体及亲和填料：
蛋白质甲基化、乙酰化、磷酸化， 生物素化，赖氨酸羧甲基化（老化糖化蛋白），半胱氨酸化，及半胱氨酸羧甲基化 等翻译后修饰蛋白质组学的泛特异性抗体及其用于纯化富集的抗体Agarose亲和填料！
2：修饰蛋白/多肽蛋白质组学研究服务：
提供蛋白质修饰多肽位点的蛋白质组学测序服务，修饰蛋白/多肽差异 表达的质谱分析。申请生理病理状态下修饰的蛋白研究的课题提供构想！
温馨提示：不可用于临床ZL。
