The binding affinity of anti-biotin antibodies could improve the elution of biotinylated peptides. This table shows the difference in the # of unique peptides when samples are biotinylated.
Biotin antibody compared to non-biotin samples
Immunechem’s Biotin Antibody Agarose (ICP0615) yielded the highest number of biotinylated peptides
compared to Sigma-Aldrich and Santa Cruz.
For more details, please refer to: Nature Methods
Volume 14, Issue 12, 16 October 2017, Pages 1167–1170