DMEM, powder, high glucose, pyruvate
Catalog number: 12800017
Related applications:
DescriptionDMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the .
This DMEM is modified as follows: | With | Without |
| • High Glucose | • HEPES |
| • L-glutamine | • Sodium Bicarbonate |
| • Phenol Red | |
| • Sodium Pyruvate | |
The complete is available.Using DMEMDMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco® cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see for details).cGMP manufacturing and quality systemDMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable DMEM product made in our Scotland facility (12800-116). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
SpecificationsGlutamine:Phenol Red Indicator:Form:Glucose:Serum Supplementation:HEPES Buffer:Sodium Pyruvate Additive:Product Line:Product Size:Sodium Bicarbonate Buffer:Shipping Condition:
| L-Glutamine |
| Phenol Red |
| Powder |
| High Glucose |
| Standard Serum Supplementation |
| No HEPES |
| Sodium Pyruvate |
| Gibco® |
| 10 x 1 L |
| No Sodium Bicarbonate |
| Room Temperature |
Contents & storage
Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
GIBCO DMEM高糖培养基 粉末 12800017 Gibco代理
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D6429 Sigma DMEM高糖型培养基西格玛D6429
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