DescriptionDMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the .
This DMEM/F-12 is modified as follows:
| With | Without |
| • L-glutamine | • Sodium Bicarbonate |
| • HEPES | |
| • Phenol Red | |
The complete is available.Using DMEM/F-12DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco® cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see for details).cGMP manufacturing and quality systemDMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable DMEM/F-12 product made in our Scotland facility (42400-010). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Specifications
Glutamine:Phenol Red Indicator:Form:HEPES Buffer:Sodium Pyruvate Additive:Serum Supplementation:Glucose:Product Line:Product Size:Shipping Condition: | L-Glutamine |
| Phenol Red |
| Powder |
| HEPES |
| Sodium Pyruvate |
| Standard Serum Supplementation |
| High Glucose |
| Gibco® |
| 10 x 1 L |
| Room Temperature |
Contents & storage
Storage conditions: 2-8°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Media formulations
GIBCO DMEM/F12 粉末培养基
大豆酪蛋白消化培养基，无菌粉末
21127-022F-12K进口培养基报价，热电Gibco培养基总代理
胎牛血清|细胞培养基 细胞培养gibco
1640培养基 1640 Medium价格 GIBCO货号：31800-022 规格：10X1L原装
M199培养基 M199 Medium价格 GIBCO货号：31100035 规格：10X1L原装
现货DMEM培养基高糖 C11995500BT 500ml GIBCO 价格
M199培养基 M199 Medium现货GIBCO货号：31100035
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