特别声明:本产品及我公司所售其他产品均为科研类试剂产品,严禁用于药物、YL及其他非科研用途。
北京百奥莱博专业生产销*(代"售")北京BL21(DE3)感受态细胞(pTf16分子伴侣)价格厂家,我公司供应的分子生物学试剂品类齐全,且具有优势价格,欢迎新老客户垂询订购。
名称:北京BL21(DE3)感受态细胞(pTf16分子伴侣)价格厂家
英文名:Chaperone competent cells pTf16/BL21(DE3)
规格:100μl×10
产地:国产|进口
品Pai:百奥莱博
外源蛋白质在大肠杆菌中的表达常形成包涵体或发生蛋白酶降解。大多是由于所表达的蛋白未能正确折叠造成。分子伴侣协同作用,可以帮助外源蛋白正确折叠。不同外源蛋白需要的分子伴侣不同。百奥莱博提供5种表达常用分子伴侣的BL21(DE3)衍生菌株,适用于不同外源蛋白在大肠杆菌中有效折叠。该系统适用于pET系列等依赖T7启动子的表达载体。本感受态细胞无污染,转化效率可达107以上
图例一)Human种属的RORG(31KD)在大肠杆菌中获得可溶表达。
Lane 1:上样
Lane 2: 流穿
Lane 3: 50mM Imidazole洗脱
Lane 4: 500mM Imidazole洗脱
Lane 5: 蛋白Marker
我公司的北京BL21(DE3)感受态细胞(pTf16分子伴侣)价格厂家,性价比高,货期短,送货上门,欢迎您来电咨询购买,另外,我公司还生产供应销*(代"售")下列产品:
·重组人DUSP3
编号:JN1781
英文名称:Recombinant Human Vaccinia Virus VH1-related Phosphatase
规格:10μg|50μg|500μg|1mg
本品由我们的大肠杆菌表达系统制备而成,目的基因编码的Ser2-Pro185在N端含有His标签。
DUSP3质量控制:>95%(还原性SDS-PAGE)
DUSP3制剂:液体
DUSP3保存:收到货后请置于-20℃,可保存6个月,避免反复冻融。
关于DUSP3:
Human DUSP3 belongs to the dual specificity protein phosphatase subfamily. DUSPs are a heterogeneous group of protein phosphatases that can dephosphorylate both phosphotyrosine and phosphoserine/phosphothreonine residues within the one substrate. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. DUSPs are major modulators of critical signalling pathways that are dysregulated in various diseases. They negatively regulate members of the mitogen-activated protein kinase superfamily, which are associated with cellular proliferation and differentiation. DUSP3 is expressed in human tissues including breast and ovarian.DUSP3 shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Human DUSP3 specifically dephosphorylates and inactivates ERK1 and ERK2.
北京BL21(DE3)感受态细胞(pTf16分子伴侣)价格厂家关键词:BL21(DE3)感受态细胞(pTf16分子伴侣),Chaperone competent cells pTf16/BL21(DE3),JN0128
·重组人泛素结合酶E2-V1(UBCE2V1)
编号:JN1030
英文名称:Recombinant Human Ubiquitin-Conjugating Enzyme E2 Variant 1
规格:10μg|50μg|500μg|1mg
本品由我们的大肠杆菌表达系统制备而成,目的基因编码的Ala2-Asn147在C端含有His标签。
UBE2V1质量控制:>95%(还原性SDS-PAGE)
UBE2V1制剂:液体
UBE2V1保存:收到货后请置于-20℃,可保存6个月,避免反复冻融。
关于UBE2V1:
Ubiquitin-Conjugating Enzyme Variant 1a (UBE2V1) is a member of the Ubiquitin-conjugating (E2) enzyme family. The E2 catalytic core domain of UBE2V1 lacks an active site cysteine residue, rendering it catalytically inactive on its own. However, in the cytoplasm UBE2V1 is able to form a catalytically active complex with UBE2N/Ubc13, which mediates the synthesis Lys63-linked Ubiquitin chains and is required for NF-kappa B activation. UBE2V1 is required for UBE2N (Ubc13)/UBE2V1 Complex-dependent Lys63-linked Ubiquitin chain formation. More specifically, UBE2V1 orients the Ubiquitin molecule to favor linkage at Lys63 via a non-covalent interaction with the Ubiquitin molecule. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. UBE2V1 plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6, and TRAF2. It mediates transcriptional activation of target genes. UBE2V1 also controls the progress through the cell cycle and differentiation, the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.
北京BL21(DE3)感受态细胞(pTf16分子伴侣)价格厂家关键词:BL21(DE3)感受态细胞(pTf16分子伴侣),Chaperone competent cells pTf16/BL21(DE3),JN0128
·重组人RPS7(40S核糖体蛋白S7)
编号:JN1216
英文名称:Recombinant Human 40S Ribosomal protein S7
规格:10μg|50μg|500μg|1mg
本品由我们的大肠杆菌表达系统制备而成,目的基因编码的Met1-Leu194在C端含有His标签。
RPS7质量控制:>95%(还原性SDS-PAGE)
RPS7制剂:液体
RPS7保存:收到货后请置于-20℃,可保存6个月,避免反复冻融。
关于RPS7:
40S ribosomal protein S7(RPS7) belongs to the S7E family of ribosomal proteins. It is phosphorylated by NEK6 during post-translational modification. RPS7 is located in the cytoplasm, binds IPO9 with high affinity. it also can interacts with NEK6. As is required for rRNA maturation and typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. The unnormal expression of RPS7 may cause Diamond-Blackfan anemia 8.
北京百莱博科技有限公司专业生产供应销*(代"售")生物试剂、化学试剂、抗原抗体、血清血浆、Elisa试剂盒,更多试剂产品需求,欢迎来电咨询选购北京BL21(DE3)感受态细胞(pTf16分子伴侣)价格厂家。
温馨提示:不可用于临床ZL。