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shRNA载体构建 干扰质粒靶点设计
1,干扰质粒靶点设计设计三条(一般构建三条)+一条NC 一套2000元
2,载体选择
瞬转质粒: 质粒详细信息 | pHHsi-H1-EGFP-puro 瞬转shRNA质粒RNAi |
质粒详细信息 | pSilencer 2.1-U6 hygro RNAi系统载体 shRNA |
质粒详细信息 | pHHsi-H1-EGFP-puro 瞬转shRNA质粒RNAi |
质粒详细信息 | pGPU6/GFP/Neo 绿色荧光蛋白 shRNA载体 |
质粒详细信息 | pSUPER.neo+GFP 哺乳细胞RNAi载体 shRNA H1启动子 |
质粒详细信息 | pSilencer 4.1-CMV neo |
慢病毒质粒: 质粒详细信息 | pHHLV-H1-ZsGreen1慢病毒shRNA干扰载体 绿色荧光 |
质粒详细信息 | pHHLV-H1-mCherry-T2A-BSD慢病毒干扰载体 红色荧光 Blast |
质粒详细信息 | pHHLV-H1-BSD慢病毒shRNA干扰载体 Blast杀稻瘟菌素 |
质粒详细信息 | pLVX-ShRNA2-Puro-NC 慢病毒shRNA对照质粒 zsgreen puro |
质粒详细信息 | pSIH1-H1-copGFP-T2A-Puro-NC 慢病毒系统对照载体 shRNA |
质粒详细信息 | pLVX-ShRNA2-Puro 慢病毒shRNA,RNA干扰载体 zsgreen puro |
质粒详细信息 | pHHLV-H1-copGFP-T2A-Puro 慢病毒shRNA载体 GFP Puro |
质粒详细信息 | pHHLV-H1-mCherry 慢病毒shRNA载体 RNAi载体 mcherry |
质粒详细信息 | pLVX-shRNA1 慢病毒shRNA,RNA干扰载体 嘌呤霉素 puro |
质粒详细信息 | pLVX-shRNA2 慢病毒shRNA,RNA干扰载体 zsGreen 绿色荧光 |
质粒详细信息 | pHHLV-H1-Puro慢病毒shRNA干扰载体 嘌呤霉素 |
质粒详细信息 | pLVX-shRNA1 慢病毒shRNA,RNA干扰载体 嘌呤霉素 puro |
质粒详细信息 | pLVX-shRNA2 慢病毒shRNA,RNA干扰载体 zsGreen 绿色荧光 |
质粒详细信息 | pLKO.1-EGFP-puro 慢病毒干扰质粒 绿色荧光蛋白 嘌呤霉素 shRNA |
质粒详细信息 | pLKO-Tet-ON 诱导型慢病毒干扰载体shRNA嘌呤霉素筛选 |
腺病毒质粒:
腺相关病毒质粒:pAAV-zsgreen-shRNA
逆转录病毒质粒:PMKO.1-Puro,RNAi-Ready pSIREN-RetroQ 质粒名称
pCDH-CMV-MCS-EF1-CopGFP-T2A-Puro(CD513B)
pCDH-CMV-MCS-EF1-Puro (CD510B)
pCDH-EF1-MCS-T2A-Puro (CD520A)
pCDH-CMV-MCS-EF1-RFP-T2A-Puro(CD516B-2)
pLVX-EGFP-IRES-Puro(pLVX-IRES-Puro改)
pLenti6-V5-MSC(pLenti6-V5-D-TOPO改)
pLVX-IRES-ZsGreen1
pLVX-mCherry-N1
pLL3.7
pGIPZ
pSicoR
psPAX2
pMD2.G
pLP1
pLP2
pVSV-G
pLP-VSVG
pMDLg/pRRE
pRSV-Rev
pHHsi-hU6-GFP-Puro
pLVX-DsRed-Monomer-N1
pHR-SFFV-dCas9-BFP-KRAB
pQKX2
lentiGuide-Puro
plvx-GFP-LC3
pLVX Tre3G
pLVX-Tet3G
pLV-N-Flag-mCherry-IRES-puro
pLVX-TetOne-Puro
pCDH-CMV-MCS-EF1a-GFP+BSD
pCDH-CMV-MCS-EF1a-mcherry+BSD
pLVX-NEO-IRES-tdTomato,PLVX-IRES-tdTomato-NEO
pLVX-IRES-tdTomato
pLenti-Bi-cistronic
pCDH-EF1-Luc2-T2A-TdTomato
pCDH-MSCV-MCS-EF1-copGFP-T2A-Puro
pCDH-EF1a-MCS-T2A-copGFP CD521A-1
pCDH-CMV-mRFP-GFP-hLC3B-EF1A-Puro 自噬双标慢病毒载体
pLenti-CMV-V5-Luc-Blast (w567-1)
pLenti-CMV-GFP-puro
plenti-CMV-Puro-Luc
plvx-tetone-luc 自己插入的luc
plvx-mCMV-ZsGreen1-Puro
pCMV-dR8.91
PiggyBac Dual Promoter PB513B-1
PLVX-IRES-PURO
plvx-puro
pLEX-Flag-MCS
pRE112/X7213
pKD46
pCP20
pwtCas9-bacteria #44250
pC001-huLshC2C2-MBP 79150
pCas9 #42876
pCAS #62225
pTargetF #62226
pCRISPR #42875
pJ23-sgRNA
pX260 #42229
pX330 #42230
pX335 #42335
pX458 #48138
pX459 #48139
pX458M(+EZ-GuideXH)
pX459M(+EZ-GuideXH)
EZ-GuideXH
pX461 #48140
pX552 #60958
pX601 #61591
pX602 #61593
LentiCRISPR V2 #52961
pLenti dCasVP64-Blast #61425
pDD162 (Peft-3::Cas9 + Empty sgRNA) #47549
pmCherry-C1-EGFP(CRISPR验证质粒)
pY010 (pcDNA3.1-hAsCpf1) #69982
pY011 (pcDNA3.1-hLb2Cpf1) #69973
pY016 (pcDNA3.1-hLbCpf1) #69988
pY004 (pcDNA3.1-hFnCpf1) # 69976
AIO-mCherry #74120
lentiGuide-Puro(Plasmid #52963)
pHR-SFFV-dCas9-BFP-KRAB #46911
pLV hUbC-VP64 dCas9 VP64-T2A-GFP 59791
Plasmid53186 phH1-gRNA
Plasmid53187 pmU6-gRNA
Plasmid53188 phU6-gRNA
Plasmid53189 ph7SK-gRNA
pJ23sgRNA-eGFP 3
Cas9-NAT酵母 #64329
Cas9-NAT-kan酵母
pdCas9-Tet1-CD #83340
pET-NLS-Cas9-6xHis(Plasmid #62934) 原核表达CAS9蛋白
lentiCRIPSR-V2-GFP (puro替换为GFP )Cas9-GFP sgRNA CRIPSR-V2
pHAGE-TRE-dCas9-KRAB #50917
lenti sgRNA(MS2)_zeo backbone 61427
pcDNA-dCas9-VP64 #47107
pST1374-NLS-flag-linker-Cas9 #44758
pGL3-U6-sgRNA-PGK-puromycin #51133
pKMD106e-intein-Cas9(S219) #64190
lentiMPH v2 (Plasmid #89308)
lentiSAMv2 (Plasmid #75112)
pSLQ1651-sgTelomere(F+E)#51024
PHAGE-To-dCAS9 #75381
PX459.2.0 #62988
eSpCas9(1.1) #71814
pCDNA-dCas9-p300Core
lenti sgRNA(MS2)_puro backbone Plasmid73795
lenti MS2-P65-HSF1_Hygro Plasmid61426
pCDNA-dcas9 p300core(Plasmid #61357)
pX462 # 48141
pGH335_MS2-AID Δ-Hygro-85406
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP-60903
pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS-60904
pET302-6His-dCas9-Halo #72269
pGL3-Basic
pGL3-Promoter(未验证正确)
pGL3-enhancer
pGL3-Control
pRL-TK
pRL-SV40
pGL4.10[luc2]
pGL4.18[luc2PNeo]
pGL4.20(非空载,插入68碱基)
pGL4.22
pGL4.23 [luc2minP]
pGL4.31
pGL4.74[hRluc/TK]
pGL4.75[hRluc/CMV]
psiCHECK-2
pmirGLO
pMIR-Reporter-Luc
pCLucIPZ 53222
pCMV-Gluc
pGL4.31
pSV-Beta-Gal
pGL4.19
pNL3.1
pNL2.1
pNL1.2
pNFKB-LUC
PSBE-LUC
CHOP promoter (-649/+136) pmCherry-1(Plasmid 36035)
pNF-kB-TA-Luc
PG5luc非空载 酶切位点破坏
M50 Super 8x TOPFlash(Plasmid #12456)
M51 Super 8x FOPFlash (TOPFlash mutant)(Plasmid #12457)
pGL4.51
pAP1-Luc
pGL4.33[Luc2p-SPE-Hygro]
psicheck2 VEGFA 3'UTR
ptfhLC3B人
ptfLC3大鼠
pCDNA3.1(+)-3HA-hLC3B人
plvx-GFP-LC3人
pCDNA3.1(+)-hLC3B人
pEGFP-LC3人
pCDNA3.0
pCDNA3.0-flag
pCDNA3.1(+)
pCDNA3.1(+)-3HA
pCDNA3.1-Myc-HisB
pcDNA3.1(+) CircRNA Mini Vector(Plasmid 60648)
pcDNA6-myc-His A
pcDNA6-V5-His-LacZ
pIRES2-EGFP
pIRES2-zsGreen1
pTRACER-CMV3
pVAX1
pCEP4
pCEP4-CAT
pBudCE4.1
pBudCE4.1/lacZ/CAT
PCI-neo
pEGFP-C1
pEGFP-N1
pEGFP-MHF
pDsRed1-N1
pmcherry-c1#
pRI-GFP/hygeo
pCXWB-EBNA1
pmR-mCHerry
pTT5
pTagBFP-N vector
p3XFLAG-CMV-7.1
pCS2 Notch1 Full Length-6MT(Plasmid #41728)
pMy-BirA-T2A-eGFP
pBIFC-bFosVC155
pBIFC-bJunVN173
pBIFC-VC155
pBIFC-VN173
pCDA3.3(pCDA3.3-TOTO上添加酶切位点)
pCDA3-5'FLAG(pCDA3.0添加Flag(DYKDDDDA))
p3XFLAG-CMV-14
pCDNA3.1-FLAG
pCDNA4.0-Myc-His A
pCDNA4.0-V5-His
pBiFC-VN155 (I152L)
pBiFC-bJun-VN155(I152L)
pBiFC-bFos△ZipVC155(与Plasmid有一个氨基酸差异)
pActin-Actin
pCAGGS
pcDNA6.2-GW-EmGFP-miR载体(非空载)
pECFP-N1
pCDNA3.1(-)
padvantage
pDsRED2-Mito
pDsRED-Monomer-N1
HA~GLUT4~GFP
pBIFC-CrN173
pBIFC-CC155
pEGFP-LC3人
pCI-neo-GFP
pEYFP-Actin
FLAG-CMV-6a
pDsReD1-C1
pires
RNAi-Ready pSIREN-RetroQ
pEGFP-LC3B mice
pmCherry-EGFP-mLC3B
pbind
pACT
PCMV5-HA
pEE6.4
pTet-off-advanced
pIRES-Puro3
pFUSE-hIgG1e1-Fc2
pFUSE-hIgG1-FC2
pmCherry-EGFP-mLC3B
PEE12.4
pDisplay
pFUSE-hIgG4-Fc2
pmVenus-C1
pNTAP-B
PCDNA3.1-EGFP
pXC17.4
pXC18.4
pCHO1.0
pFUSEss-CHIg-mG1
pFUSEss-CHIg-mG2A
pFUSEss-CHIg-mG2B
pFUSE-mIgG1-Fc2
pCGS3
pHHCMV-C-Flag(pVAX1-C-Flag)
pHHCMV-C-HA(pVAX1-C-HA)
pHHCMV-C-myc(pVAX1-C-myc)
pHHCMV-N-Flag(pVAX1-N-Flag)
pHHCMV-N-HA(pVAX1-N-HA)
pHHCMV-N-myc(pVAX1-N-myc)
pHHCMV-C-twinSTREP
pSilencer 4.1-CMV neo
pLKO.1-EGFP-Puro
pLKO.1-EGFP
pLKO.1-Puro
pLKO.1-TRC cloning vector
pLVX-shRNA2
pLKO-Tet-ON 或Tet-pLKO-puro
pSUPER.neo+GFP
pSUPER.neo+GFP-shLUC(对照NC)
pMXs-miR-GFP Puro
pAAV-zsgreen-shRNA(非空载,插入shSnail,酶切位点未破坏)
pLKO.1-GFP-CAMP
pLKO.1-Puro-CAMP
pLKO.1-GFP-DEFB4A
pLKO.1-Puro-DEFB4A
pLKO.1-GFP-TBX21
pLKO.1-Puro-TBX21
pLKO.1-GFP-EOMES
pLKO.1-Puro-EOMES
pLko.1-GFP-shLuc
pLKO.1-mRFP
pLKO.1-RFP-shLuc
psuper
pSIH1-H1-mCherry
PMKO.1-Puro
Psuper-retro-puro-shNur77
METTL14-shRNA1
METTL14-shRNA2
METTL14-shRNA3
FTO-shRNA1
FTO-shRNA2
FTO-shRNA3
ALKBH5-shRNA1
ALKBH5-shRNA2
ALKBH5-shRNA3
METTL3-shRNA
YTHDF1-shRNA
YTHDF2-shRNA
pLKO-puro-shP2X7R-1
pLKO-puro-shP2X7R-2
pLKO-puro-shP2X7R-3
pSIH1-H1-copGFP-T2A-Puro
PLVX-shRNA1
pGEX-4T-1
pGEX-4T-2
pGEX-4T-3
pGEX-6P-1
pET-3a
PET9A
pET-17b
pET-20b
pET-21a(插入片段 乙肝病毒序列)
pET-22b
pET-28a
pET-28b
pET-30a
pET-32a
pET-32c
pET39b
pET-42a
pET-52b-sfGFP
pQE-30
pQE-31
pQE-32
pQE-60
pQE70
pColdI
pColdII
pColdIII
pColdTF
pEX18Tc (测序显示在非结构区多23bp)
pMBP-C His
pACYC184
pACYCduet-1
pRSF Duet-1
pREST b
pBV220
pEZZ18
pMAL-p2x
pPSUMO(pET28插入sumo)
pTrc99a
pET-His
pET-GST
pKK233-2
pSR43.6r
pWUR702 53079
pCold-SUMO
pGEX-HRV3C
pet24a
pGEX-6P-1-dtomato 原核表达粉色蛋白
pET28-sfGFP原核表达绿色蛋白
pET28-tdtomato原核表达粉色蛋白
pGEX-6P-1-sf-GFP原核表达绿色蛋白
pET-21a
pET29a
pMAL-C2x
pTrcHisA
PETDuet-1
pGEX-6P-2(非编码部位有突变)
pGEX-6P-3(Amp部位有突变)
pGEX-5X-1
pGEX-5X-3
pET-25b-SBP (NB6)
pColADuet1
pTwin1
pTrcHis2B
PET28a-EYFP(Citrine)
pET-28a-IL18
pET-28a-TNNT2
pET-28a-βNGF
pET-41a-CTNI
pG-KJE8 氯霉素
pGRO7 氯霉素
pG-TF2 氯霉素
pKJE7 氯霉素
pTf16 氯霉素
pGH(small)HCST
PET23B
pMAL-c5x
pET28b-CAS9-his
pGEX-2TK
pET-4CDS
pFastBac1非空载(PBS1拟南芥)
pCoHygro
pFastBac HT-B
pFastBac Dual
pFastBacHT-CAT
pDEST10
pCoBlast
pCoPURO
pPIC9
pPIC9k
pPICZα A 测序正确
pGAPZB
pAO815
pAbAi
pYES2.0
pYES2/CT
pGADT7
pGADT7-T
pGBKT7
pGBKT7-53
pGBKT7-lam
Cas9-NAT #64329
Cas9-NAT-kan酵母
pBridge
pUG6
pESC-Ura
pPH37
ppinkα-hc
pYes2/CT α-factor
pSH65
pPR3-C
pDUAL2-FFH1 测序正确一个碱基差异不影响FLAG
pDUAL2-GFH1 测序正确,GFP FLAG两个碱基差异
pDUAL2-HFG1 测序正确 GFP一个碱基差异
pDUAL-FFH1 测序正确一个碱基差异不影响FLAG
pDUAL-GFH1 测序正确一个碱基差异不影响FLAG
pAN7-1
pYES3/CT
pSH47
pESC-HIS
pESC-LEU
pESC-TRP
pBABE-HA-puro
pBABE-LUC+
LZRS-HOTAIR
LZRS-control
pLEGFP-N1
pCMV-VSVG
pMSCV-PIG
pMSCV-NEO-luciferase
pMXs-miR-GFP Puro
pMSCV-loxP-dsRed-loxP-eGFP-Puro-WPRE
pMRX-IP-GFP-LC3-RFP #84573
pMRX-IP-GFP-LC3-RFP-LC3ΔG #84572
pLNCX2
pQCXIH
pQCLIN
pLPCX
pLAPSN
pMSCVhyg
pLEGFP-C1
pMSCV-IRES-GFP II(Plasmid52107)
pAAV-MCS
pHelper
pAdEasy-1
pShuttle
pAdBLOCK-iT-DEST(验证有ilr但是和原序列不一致)
pAAV-RC-9(是血清9型,非网上序列)
pShuttle-IRES-hrGFP-2
pAAV-zsgreen-shRNA(非空载,插入shSnail,酶切位点未破坏)
pAdtrack
aav2/8
pAAV-IRES-EGFP(由pAAV-MCS改造)
pacad5-9.2-100
pacad5-cmv-npa
pacad5-mir-GFP
pDC316-mCMV-EGFP
pDC316-ZsGreen-shRNA(PstI,BamHI)
aav2/2 有测序,没有模板比对
aav2/5 有测序,没有模板比对
pAAV-CAG-GFP(多13个碱基,不影响)
pAdTrack-CMV
pDC316-mCMV-tdTomato
pDC316-mCMV-GFP
pDC316-EGFP-shRNA(PstI,BamHI)
pDC316-tdtomato-shRNA(没有比较好的酶切位点)
pBHGlox-△E1,3cre
pdc316
pBRIT-HA/FLAG
PUMVC
pDC316 mCMV tdTomato EGFP
pDC316-tdtomato-shRNA-AgeI
pshuttle-cmv cre 预测
pShuttle-IRES-hrGFP-1
pAAV-RC2
pAdeasy-IGF1(未验证正确)
pAdeasy-EGFP(未验证正确)
AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Plasmid51084
padDeltaF6
phelper(sui)
paav-rc9(sui)
pAAV-RC5
pDonr201
pDonr207
pDONR221
pEarleyGate 201(N-HA)
pEarleyGate 301(C-HA)
pEarleyGate 302(C-FLAG)
pEarleyGate 303(C-Myc)
pDEST15
Plenti-CMV-puro-Dest(Amp)
pAG306GPD-EGFP-ccdB
pGM3301(吉林农科院)
PGR107
pJL-TRBO Plasmid80082
pB7WG2
pSAT6-nEYFP-N1_(E2913)
pSAT6-nEYFP-C1_(E2884)
pSAT6A-cEYFP-N1
pSAT6-cEYFP-C1-B
pKEX4tr 氨苄抗性
pLSLR Plasmid51500
pK7WGWI(GI)(未验证正确)
pGreenII 0800-LUC
pBI121
pCAMBIA0380
pCAMBIA1391(测序在潮霉素基因启动子部分少两个碱基,但功能没有问题)
pCAMBIA3300
pcambia1300(在多克隆位点前有突变,表达没有影响)
pCAMBIA1304
pRI101-AN
pRI201-ON
pBUE411
pCUNm-GFP
pS1300
pCBC-mT1T2
pRI101-AN
pRI201-ON
pGreenII 62-SK
EX-NEG-M03
pHY300PLK
pK18mobsacB
pDGICZ
PMK3
pDG1730
pL-scFv(ch14.18)HL-zeta-SN (CAR-T相关)FH3948
pGIPZ
pMA2775 Plasmid46884
pCDH-ciR
pMSCG7(pMCSG7)
pMSCG19(pMCSG19)
pMV306hsp+LuxG13 #26161
pMV306hsp+Lux #26159
pBBRMCS-2
pBE2RS(pBE2-R)
pK19mobsacB
pME6032
pP43NMK
pRK415
psilent-dual1(pSD1)
pHT304
ppyCAGIP-AR(人源)
pTRV2
pVLT33
pAN8-gpdA(非pAN8-1)
PHW2000
pMA5 不知怎么测序
pGM1190(与Plasmid69994有区别,多克隆位点区经过标签改造)
pcomb3x
pcomb3x-ss
pcomb3x-lamda
pCDNA3.1-circRNA
pCD2.1-ciR(不好测序)
pLCDH-CIR(不好测序)
pLCDH-CIR-yang
pNL4.3
pEC-XK99E
pJYS1Ptac(Plasmid85545)
pJYS2-crtYf(Plasmid85544) 酶切正确
pJYS3-△crtYf(Plasmid85542) 酶切正确
pBAD18
pBAD18-Cm
pBAD18-Kan
pHT43
pGfa2-vehicle
pGfa2-nlac
pNZ8148
pHEN1
pBAD33
pCY 3010-07(Plasmid 36212),用T7测序发现有些点突变,但功能无影响)
CD109-bio-His (Plasmid #51862)
pBABE-hLin28B #26358
pLVX-AcGFP-N1-h-TP53
pCDNA3.1(+)-h-STAT3
p3×Flag CMV-10-h-Oct4
pMXs-hSOX2 (#17218)
pMXs-hKLF4 (#17219)
pLentG-KOSM
pMXs-hOCT3/4
pMXs-hc-MYC
pDest27 EGFR 没有测通
pCDNA3.1(+)-h-TCF7L2
pCMV5 HA Smad4 可能是小鼠的
pCDNA4/Full length FVIII(Plasmid #41036)
pLVX-EGFP-LIN28A 人(标签名pLVX-LIN2PA)
pLVX-rTP53(大鼠)
pdonr223-Mindbomb E3(插入基因)
EX-W0858-M98 Seq(Homo sapiens(HES1), mRNA)
pCDH-EF1-MCS-T2A-Puro-Hes1(NM_008235)
PCDNA3.1-PRGG1(NM_000950.2)差异一个碱基
Pcmv-myc-AMPKα1
Pcmv-myc-AMPKα2
pEGFP-C1-LXRα
pEGFP-C1-RXRα
pEGFP-N1-Nur77
pEGFP-N1-RARgama
PCMV-myc-SP1
pCMV6-XL5-NQO1(oriGENE)
Flag-SIRT1 (Plasmid 1791)
SIRT2 Flag (Plasmid 13813)
SIRT5 Flag (Plasmid 13816)
pCMV6-AC-SIRT7(NM_016538)(oriGENE)
cmv-ub(wt)(串联的HA-ub)空载未知
pCMV-myc-HBX
PCDH-GFP-RGMB
PCMV-MYC-His-HSP70
pFLAG-CMV2-p85a(AGM7, GRB1, IMD36, p85, p85-ALPHA)
PCMV-MYC-STC2
pRK5-HA-Ubiquitin-WT (Plasmid #17608)
pCMV—HA-ubiquitin(WT) (骨架不对)
pCMV—HA-ubiquitin(K48R)
pCMV—HA-ubiquitin(K63R)
atg5
atg6-gfp
atg8
atg12
"pCDNA3.1(+)-flag-RAB8(NM_005370.4)
(同名MEL, RAB8A)"
pcDNA3.1-EGFP-Rab7 WT (差异一个碱基,不影响氨基酸)
pEYFP-N1-Lamp1(NM_012857.2)大鼠 差异一个氨基酸
HA-HIF1A(MOP1;PASD8;bHLHe78)(骨架pBABE-puro)
FLAG-HIF2A (ECYT4;EPAS1;HLF;MOP2;PASD2;bHLHe)(骨架CD510B)(GenBank: BC051338.1)
pcdna3.1-HA-smad1
pCDNA3.1(-)Myc-HisB-NR2B 别名GRIN2B
HPS1 FL (空载未知,可能为pAG?)
HPS2-PCDNA3.1
pCMV3-c-myc-GOT1-(glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)谷草转氨酶1)
plenti-GFP-rab5(空载不清楚)
pCMV-Myc-P62(一个碱基突变不影响氨基酸)
pET28a-ECFP
pET28a-mCherry (多一个碱基,有移码突变)
pmCherry-Tubulin-C-18(Plasmid 有部分序列)
HDAC1 (pCDNA3.1)
pet28a-GAPDH(大肠杆菌)(glyceraldehyde-3-phosphate dehydrogenase WP_063087739.1)
hPS1非CMV启动子 (CAG启动子?)
pCDNA3.1-hPS2
pCDNA3.1(-)Myc-HisB-NR2B 别名GRIN2B
pCDNA3.1(+)-Nluc2
pCDNA3.1(+)-Cluc2
pCDNA3/TO-pepANluc2BCluc2 3×caspase3
pEGFP-C1-Beclin I (human)
pEGFP-N1-Beclin I (human)
pCDH-DRAM1-③
pCDH-DRAM1-④
pCDNA3.1-EGFP-PD-L1(胞外段)
pYD5-PD1
PFFH1-mcherry (mcherry-flag)
pGFH1-atg8
pCDNA3.1-CDK5
CLDN8 (NM_199328) in pcDNA3.1-3xFlag-C
SLCO3A1(NM_001145044.1) in pcDNA3.1-EGFP-C
TCF21 pcDNA3.1-Flag-C
FOXO3 pLVX-AcGFP1-N1
pCDH-TLR9(突变一个碱基不影响蛋白)
pCDH-BX647792(非编码)
pCDH-YKL-40(小鼠)
SIRT3 Flag(Plasmid 13814)pcDNA3.1 +
SIRT6 Flag(Plasmid 13817)pcDNA3.1 +
pBRPyCAG-Mettl3-dsRed-IRES-puro #61516
pCMV-SPORT6-TLR9
pCDNA-MMP13
pLV-control
pLV-wnt3a
pCDNA3.1-ICN1
pCMV-myc-nur77
pUC57-NR026860
pCMV5-3HA-AMPKα1
pCDNA3.1-3HA-HDAC4(BC039904.1)骨架 可能不是这个
C43(DE3)
C41(DE3)
DB3.1
DH5a
TOP10
JM110
JM109
JM103
stbl3
C600
XL1-blue
TG1
ER2738
GS115
Y1HGOLD
pET28a-TEV
pCas-Guide
pAmcyan-N1
pAAV-RC5
pCAG Cas9-2A-Citrine
pRPR1_gRNA_handle_RPR1
pGPU6/GFP/Neo
p414-TEF1p-Cas9-CYC1t
TetO-FUW-OSKM
FUW-M2rtTA
pCS2-nCas9n-nanos 3'UTR
pSingle-tTS-shRNA
pEX18GM
pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HANLS(s)(Plasmid 47868)
pASKIBA5plus+FUS(人基因)
pBeloBac11
pCerulean-N1
pCXLE-hOCT3/4-shp53F(Plasmid27077)
pCXLE-hSK(Plasmid27078)
pDest17
PEGFP-ER
PET28a-mCerulean
Super PiggyBac Transposase (PB200PA1)
pK19mobsacB-T7 polymerase
pMJ826(Plasmid 39316)
pMT-BiP-MCS-V5_6HisA
pY26TEFGPD
pCMV-Flag-CYFIP2小鼠
pCMV-Flag-MBNL1
pCMV-Flag-NFATc3
pCMV-Flag-RNF149 卡那
pCMV-Flag-SGPL1小鼠
pCMV-Flag-SOAT1
pENTR-mSTIM2-Myc
Plenti-GalT
pEGFP-C1-PRKAA1 #30305
pLenti-CD38-His-Flag
pENTR-N-flag-His
pLove-empty#15948
pLove-GFP#15949
pGEX4T-IL2
pLove-CD16(测序为cd38)
pLove-SOX2#15953
pLove-N.MYC#15951
pLove-OCT4
pLove-KIf4 #15950
Plox-CW-CRE #12238
pLove-GFP-TRPM2(未验证正确)
pENTR-RFP-GFP-LC3 #
pENTR-TPC2-flag-His
pENTR-CAMKI
pEGFP-N1-TFEB-#38119
pcDNA4-Vps34-flag #24398
pGEX4T-h-auroa A(未验证正确)
pLenti-RFP-PLK1
eGFP-Rabincon #28022
Myc-MTOR #1861
myc-ulk1 #27629
mCherry-Aurora A #54997(未验证正确)
pFLAG-CMV-hATG13 #22872
pCoslos-His-EGFP
pLVX-IRES-mCherry
pCL-10A1
pCL-Ampho
pCL-Eco
pGFPUv
pRevTet-Off(未验证正确)
pRevTet-On
PDP5rs
pAAV-CAG-RFP(未验证正确)
MG1655(菌)
pLVX-IRES-zsgreen-TRIM38
paav-rc8(未验证正确)
pFC332
pAAVS1-P-CAG-GFP #80491(未验证正确)
pET16B.Pfu (Plasmid12509)(管标记pET15b-pfu)
pVL1393
pECMV-3×Flag-GSK3B
PHT43 mcherry
PHT43 EGFP
pEF6/myc-His C
pSilencer 3.1-H1 neo
pSilencer 4.1-CMV puro(未验证正确)
pCambia1305.2
pSilencer 2.1-U6 hygro
pSilencer 3.1-H1 hygro(未验证正确)
pSwitch
AR pcDNA3.1
pBI-CMV1
PGRE-LUC
PCRE-LUC
pVE5523(测序结果不好)
pLV-CMV-MCS-EF1-ZsGreen1-T2A-Puro 全序列不知
pLV-CMV-MCS-EF1-mCherry-T2A-Puro 全序列不知
pcDNA3.1-Cre
pHR_PGK_antiCD19_synNotch_Gal4VP64 #79125
pX330S-2-PITCh
pCRIS-PITChv2-FBL
pLenti-EF1α-DsRed-Monomer-T2A-Puro-U6-gRNA
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP
pC-sleeping beauty
pFGC-pcoCas9
pCS2-nCAS9n
p415-PtrpC-Cas9-TtrpC-CYC1t
pC011 LwCas13a locus with targeting spacer(未验证正确)
pCAMBIA1303-TrpC-Hygro-gpdA-GFP
pHR-SFFV-KRAB-dCas9-P2A-mCherry
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro
p426-SNR52p-gRNA.csr-1.Y-SUP4t
pcU6_3 sgRNA
DR274
pSBbi-RN #60519(未验证正确)
pSBbi-GB #60520
pCAS9-mCherry empty(Plasmid #80975)(未验证正确)
pdCas9-bacteria(Plasmid #44249)
pgRNA-bacteria(Plasmid #44251)
pET21a-PP (PP酶/3C酶)
pET21a-ULP(管标CLP) SUMO protease
p415-PtrpC-Cas9-TtrpC-CYC1t(Plasmid #68059)
p426-SNR52p-gRNA.csr-1.Y-SUP4t(Plasmid #68060)
pHR_PGK_antiCD19_synNotch_Gal4VP64 #79125
PUGW
POG44
pCS2-nCas9n
PGL-4.20
PRL-CMV(未验证正确)
PRLG3A
pLV-CMV-MCS-EF1-ZsGreen1-T2A-Puro
pLV-CMV-MCS-EF1-mCherry-T2A-Puro
p415-GalL-Cas9-CYC1t(Plasmid 43804)
p426-SNR52p-gRNA.CAN1.Y-SUP4t(Plasmid 43803)
pC013-Twinstrep-SUMO-huLwCas13a(Plasmid 90097)
pC015-dLwCas13a-NF(Plasmid91905)
HDAC3基因质粒(未验证正确)
pCMVβ-p300-myc
pDestTol2CG2
pET22b-KOD(测序发现一个碱基突变,淼灵也这样)
pHR_PGK_antiCD19_synNotch_TetRVP64 #79128
pINDUCER11 (miR-RUG)(Plasmid 44363)
pnz8149(没有测序)
IKKalpha (S176E,S180E)-pcw107-V5 #64608
pcDNA3.1-Myc/His A
pcDNA3.1 Hygro(+)-Flag
pEYFP-N1
pEGFP-HA-Ubiquitin慢病毒(PCDH系列)CD511B-1
pcDNA3.1 Hygro(+)-Flag-SPOP
pcDNA3.1-FADD-Myc/His
pEGFP-C1-RBFOX2(未验证正确)
pCMV-His-KVNQ1(pHis-KV7.1)
pcDNA3.1-Sting-Myc/His
pcDNA3.1-KPNA5-Myc/His
pcDNA3.1-KPNA6-Myc/His
pRP-EGFP/Puro-hRAP1GAP-3×FLAG] 可能是慢病毒的(未验证正确)
pCambia1301
pCRCT酵母CRISPR
pcambia1302
pJCV53-12868bp
pK7GWIWG2-II-RedRoot
pcDNA3.1-APH1-β(mAph1b NM_177583.4)
plvx-IRES-mBace1
pcDNA3.1-mBace2
plvx-IRES-mGNB1
pcDNA3.1-mCASP4
pmCherry-N1-MCL1
pDC316-mRFP-GFP-LC3B大鼠
pDC316-flag-mAIM2 两个氨基酸差异
pDC316-flag-mASC (Pycard NM_023258.4)
pDC316-flag-CASP1(mCasp1 NM_009807.2)
pDC316-6His-EGFP-mLCN2
pET28a-GFP-tau(P301L)
pET15b-T4 DNA ligase
pCMV6-AC-IRES-GFP
pFGC5941
6-His-MBP-TEV-FnCpf1(Plasmid #90094)
xCas9(3.7)(Plasmid #108379)
xCas9(3.7)-ABE(7.10)(Plasmid #108382)
xCas9(3.7)-BE3(Plasmid #108380)
pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q-T375G)-delta-984-1090(#103871)
pcDNA3.1-hPdCpf1(pY018)
pRGEB32(Plasmid #63142)
pThermoCas9_ctrl(Plasmid #100981)
pYLCRISPR_Cas9Pubi-H(Plasmid #66187)
pLIX_403 (Plasmid #41395)
pK7WGF2 hCas9 (Plasmid #46965)
pCMV-ABE7.10 (Plasmid #102919)
pcmv-Myc-SENP1C603S
pcmv-Myc-PIAS2C335S
pcmv-Myc-SENP1
pcmv-Myc-PIAS2
pCold-GST
pCold-IV
pPMBP
pEGFP-C3
pEGFP-N3
pcDNA3.1-Mon1B
pcDNA3.1-RAB5
pcDNA3.1-RAB7
pcDNA3.1-RAB11A
pcDNA3.1-CAPZB
pcDNA3.1-CCZ1
pcDNA3.1-mRABgef1
pKLAC1+克鲁维酵母GG799
pHHCMV-C-Flag(pVAX1-C-Flag)
pHHCMV-C-HA(pVAX1-C-HA)
pHHCMV-C-myc(pVAX1-C-myc)
pHHCMV-N-Flag(pVAX1-N-Flag)
pHHCMV-N-HA(pVAX1-N-HA)
pHHCMV-N-myc(pVAX1-N-myc)
px458_2A_GFP_sgRNA_TIAL1 (Plasmid #106096)
px458_2A_GFP_sgRNA_TIA1 (Plasmid #106097)
pHHCMV-C-twinSTREP
pAAV-MCS-RFP-GFP-hLC3B
pcDNA3.1(-)-RFP-GFP-hLC3B
pcDNA3.1(-)-RFP-GFP-rLC3B
pDC316-RFP-GFP-hLC3B
pDC316-RFP-GFP-rLC3B
pcDNA3-TLR4-YFP(Plasmid 13018)
pGEX-4t-1-VPS26(human)
pGEX-4t-1-VPS35(human)
pJYS1Peftu(Plasmid 85546)
pLenti-CMV-MCS-GFP-SV-puro(Plasmid 73582)
pOTB7-HDAC6
pREDSIX(Plasmid 74103)
pSNAP-tag(T7)2-His
pUG66
pEYFP-C1-Akt1-NM_005163.2
pECFP-C1-TRPC1-NM_003304
pCS2-C-FLAG
pET28a-P24
pET41a-CD3e(部分序列)
PIB-V5-His
ptt5-P21
ptt5-P27
pCDNA3.1(+)-CD44
pET-28a(+)-CD9
pET-28a(+)-CD63
pET-28a(+)-CD81
pCDNA3.1(+)-RET