IMUBIND® ADAMTS13 ELISA（血管性血友病因子裂解酶ADAMTS-13酶联免疫试剂盒）

 INTENDED USE
The IMUBIND® ADAMTS13 ELISA is intended for the measurement of ADAMTS13 protein in human plasma. The assay is limited to For Research Use Only in the United States.

PRINCIPLE OF THE METHOD
Diluted plasma samples are added to microwells coated with a monoclonal antibody that binds to ADAMTS13. During an incubation period, ADAMTS13 in a sample binds to the antibody coated to the wells. Following a washing step, a biotinylated rabbit anti-ADAMTS13 polyclonal antibody is added to the microwells, which binds to ADAMTS13 protein captured on the plate during a short incubation period. A streptavidin-horseradish peroxidase conjugate (SA-HRP) is added to the microwells to complete the formation of the antibody-enzyme detection complex. Following another washing step, the addition of a perborate-3,3’-5,5’-tetramethylbenzidine (TMB) substrate and its subsequent reaction with the HRP present generates a blue colored solution. The reaction is stopped by adding sulfuric acid, which turns the solution color yellow. Measuring the solution absorbance at 450 nm and extrapolating the value with those of a standard curve determines the level of ADAMTS13 in the diluted plasma sample.