Anti-Nociceptin receptor孤菲肽受体/痛敏肽受体抗体

本公司经销Nociceptin receptor，孤菲肽受体/痛敏肽受体抗体，克隆类型为polyclonal，宿主来源是Rabbit，Nociceptin receptor孤菲肽受体/痛敏肽受体抗体可应用于WB、elisa、IP、IF、IHC等实验，欢迎垂询订购！

货号：BY-0181R
英文名称：Anti-Nociceptin receptor
中文名称：孤菲肽受体/痛敏肽受体抗体
其他名称：名Orphanin FQ receptor; Kappa-type 3 Opioid receptor; ORGC; K3 opiate receptor; KOR-3.
抗体来源：Rabbit
克隆类型：polyclonal
蛋白分子量：predicted molecular weight: 40kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, mo, pig, dog, cow, hrs, Gpig
产品介绍：Nociceptin receptor Receptor for the neuropeptide nocipeptin/orphanin FQ. Has a potential role in modulating a number of brain functions, including instinctive behaviors ａnd emotions. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase[subcellular location] integral membrane protein. Belongs to the G-protein coupled receptor 1 family.Function : Receptor for the neuropeptide nociceptin/orphanin FQ. Has a potential role in modulating a number of brain functions, including instinctive behaviors ａnd emotions. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase.Subcellular Location : Cell membrane; Multi-pass membrane protein.Tissue Specificity : In the brain, isoform KOR3 ａnd isoform KOR3C are most abundant in hypothalamus ａnd periaqueductal gray. Isoform KOR3A is highly expressed in cortex, striatum ａnd brainstem. Isoform KOR3D is highly expressed in cerebellum, hypothalamus ａnd brainstem.产品图片免疫组化（IHC）ImmunohistochemistryTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-Nociceptin receptor Polyclonal Antibody, Unconjugated（bs-0181R） 1:200, overnight at 4°C, followed by conjugation to the secondary antibody（SP-0023） ａnd DAB（C-0010） staining免疫荧光Immunofluorescence组织切片：Tissue/cell: human placenta tissue;4% Paraformaldehyde-fixed ａnd paraffin-embedded; Antigen retrieval: citrate buffer （ 0.01M, pH 6.0 ）, Boiling bathing for 15min; Blocking buffer （normal goat serum,C-0005） at 37℃ for 20 min; Incubation: Anti-Nociceptin receptor Polyclonal Antibody, Unconjugated（bs-0181R） 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3conjugated （bs-0295G-Cy3）used at 1:200 dilution for 40 minutes at 37°C. Western BlottingProtein: line1, rat heart lysates, 30ug; line2, rat brain lysates, 30ug; Primary: Anti-Nociceptin receptor （bs-0181R） at 1:200; Secondary: HRP conjugated Goat-Anti-Rabbit IgG（bse-0295G） at 1: 3000; ECL excitated the fluorescence; Predicted band size : 40kDObserved band size : 40kD