Product Description | This high affinity anti-Flg (DYKDDDDK) antibody is developed using the Flg peptide (DYKDDDDK) conjugated to KLH and affinity-purified with the DYKDDDDK peptide. The purified antibody was conjugated to FITC. The antibody is useful for detection, isolation and localization of DYKDDDDK-tag proteins. | | Western blot analysis of the cell extract with Flg-tag protein expression (lane 7 = 25 µg; lane 8 = 5 µg; lane 9 = 10 µg cell lysate) |
Species | Rabbit |
Formulation | 250 µg/mL in PBS, 50% glycerol |
Immunogen | Flg peptide (DYKDDDDK) KLH conjugates |
Conjugation | Acylation with FITC; FITC/Ab ratio - 20:1 |
Specificity | The antibody recognizes DYKDDDDK-tag proteins. |
Applications | IF (5 µg/sample) |
Scientific Description | DYKDDDDK peptide (Flag-tag) is frequently used as an affinity tag to facilitate the detection, isolation, and localization of the DYKDDDDK-fusion proteins. |
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly. |