催乳素ELISA试剂盒
INTENDED USE
This B.G TNFA ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of TNFA in the sample, this TNFA ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus TNFA concentration. The concentration of TNFA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PRINCIPLE OF THE ASSAY
This TNFA enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for TNFA. Standards or samples are then added to the microtiter plate wells and TNFA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TNFA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TNFA are added to each well to “sandwich” the TNFA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TNFA and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
REAGENTS PROVIDED
All reagents provided are stored at 2-8° C. Refer to the expiration date on the label.
1. MICROTITER PLATE 96 wells
2. ENZYME CONJUGATE 10.0 mL 1 vial
3. STANDARD.1 0pg/ml 1 vial
4. STANDARD.2 50 pg/ml 1 vial
5. STANDARD.3 100 pg/ml 1 vial
6. STANDARD.4 250 pg/ml 1 vial
7. STANDARD.5 500 pg/ml 1 vial
8. STANDARD.6 1000 pg/ml 1 vial
9. SUBSTRATE A 6.0 mL 1 vial
10. SUBSTRATE B 6.0 mL 1 vial
11. STOP SOLUTION 6.0 mL 1 vial
12. WASH SOLUTION x100 10 mL 1 vial
13. BIOTIN SOLUTION 1.0ml 1vial
14. INSTRUCTION 1
大鼠肿瘤坏死因子a
标本:血清或血浆
使用说明书
产品编号:E02T0029
本试剂盒仅供科研使用,不得用于临床及诊断使用!
预期应用
ELISA法定量检测大鼠血清或血浆中肿瘤坏死因子a的含量。
试剂组成
名称 规格 数量 保存
包被平底微孔板 96孔 1可拆卸板 2-8℃密封冷藏
酶结合物 10毫升 1瓶 2-8℃冷藏
标准品 1毫升 6瓶 2-8℃冷藏
显色剂A 6毫升 1瓶 2-8℃冷藏
显色剂B 6毫升 1瓶 2-8℃避光冷藏
终止液 6毫升 1瓶 2-8℃冷藏
浓缩洗涤液(100倍稀释) 10毫升 1瓶 2-8℃冷藏
生物素 1ml 1瓶 2-8℃冷藏
使用说明书 1份