RNA结合蛋白LIN28B抗体 Anti-Lin-28B

RNA结合蛋白LIN28B抗体Anti-Lin-28B的产品说明：
--产品介绍：Acts as a suppressor of microRNA （miRNA） biogenesis by specifically binding the precursor let-7 （pre-let-7）, a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.Subcellular Location : Cytoplasm. Nucleus. Predominantly cytoplasmic at G1 phase, accumulates in the nucleus in S and G2 phases. The frequency of nuclear localization in S and in G2 phases is 60% and 30%, respectively.Tissue Specificity : High expression in testis, fetal liver, placenta and in hepatocellular carcinoma （HCC）. Isoform 1 is only detected in moderay and poorly differentiated HCC tissues and placenta （at protein level）. Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues （at protein level）.Similarity : Belongs to the lin-28 family.Contains 2 CCHC-type zinc fingers.Contains 1 CSD （cold-shock） domain.Subunit : nteracts with ZCCHC11/TUT4.
--该抗体来源于：（Rabbit）
--RNA结合蛋白LIN28B抗体的参数介绍：
1、浓度：1mg/1ml
2、交叉反应：hu, mo, rat, chk, pig, hrs, cow, shp, Rb
3、产品类型：一抗
4、研究领域：肿瘤细胞生物免疫学表观遗传学
5、蛋白分子量：predicted molecular weight: 27kDa
6、性状：Lyophilized or Liquid
7、免疫原：KLH conjugated synthetic peptide derived from human Lin-28B
8、亚型：polyclonal
9、纯化方法：affinity purified by Protein A
10、储存液：Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
11、产品应用：WB=1:100-500ELISA=1:500-1000IP=1:20-100IHC-P=1:100-500IHC-F=1:100-500IF=1:50-200 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
12、保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

（1mg/1mlRNA结合蛋白LIN28B抗体Anti-Lin-28B，的相关产品：免疫组化常用试剂免疫印迹常用试剂各种标记二抗）