慢病毒载体是将遗传物质传递到大多数细胞包括难转染和未分化细胞中的理想工具。慢病毒颗粒在转入细胞后将转入基因整合到宿主基因组,造成转入基因的持续稳定表达。尽管如此,生产、浓缩、滴定得到高滴度、批间重复性好的慢病毒颗粒需要足够的实验经验,且耗费大量的时间。GeneCopoeia(复能基因)专业的科研人员能为您提供高质量、高滴度的慢病毒颗粒,以快速GX的方式满足您科研的需求。
GeneCopoeia(复能基因)可为您提供已包装好的纯化或未纯化慢病毒颗粒,也可提供客户定制慢病毒包装服务。高质量的质粒DNA、EndoFectin™-Lenti慢病毒转染试剂、TiterBoost™滴度增强剂及丰富的实验经验保证了病毒颗粒的高滴度。
已包装好的慢病毒颗粒 | ![]() |
已包装好的纯化或未纯化慢病毒颗粒主要包含以下方面:
已包装好的未纯化慢病毒颗粒滴度可达107 copies/ ml,纯化的慢病毒颗粒滴度可达109 copies/ ml。Lentifect™慢病毒产品均经过严格的质量控制,插入序列均经过测序验证和qPCR滴度验证。
客户定制慢病毒包装服务 | ![]() |
GeneCopoeia(复能基因)为您提供包括ORF cDNA, shRNA, miRNA前体和miRNAYZ剂在内的慢病毒包装服务。服务周期根据服务内容不同而有所差异,Z短为20个工作日,病毒滴度可达107 copies/ ml~109 copies/ ml,Z终产品规格为100 µl 或1 ml。
Lentifect™慢病毒产品优势Panel A. Numbers correspond to the volume of crude eGFP particles used.
Panel B. Numbers correspond to the volume of purified eGFP particles used.
图1. 未纯化的慢病毒颗粒LP-EGFP-LV105 (Panel A)或纯化的慢病毒颗粒LPP-EGFP-LV105 (Panel B)与5 µg/ml聚凝胺共感染24孔板中的H1299细胞。感染72小时后荧光显微镜观察eGFP表达。
图2. 未纯化的慢病毒颗粒LP-MCHR-LV105与5 µg/ml聚凝胺共感染24孔板中的H1299细胞。感染72小时后荧光显微镜观察eGFP表达。
Lentifect™已包装慢病毒颗粒 Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-EGFP-LV105-0200 | eGFP Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-EGFP-LV105-0205 | eGFP Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-EGFP-LV105-025 | eGFP Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1011 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
LP-EGFP-LV151-0200 | eGFP Lentifect Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Neo |
LP-EGFP-LV151-0205 | eGFP Lentifect Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Neo |
LPP-EGFP-LV151-025 | eGFP Lentifect Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1010 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Neo |
LP-EGFP-LV156-0200 | eGFP Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 200 µl | EF1α | Puro |
LP-EGFP-LV156-0205 | eGFP Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | EF1α | Puro |
LPP-EGFP-LV156-025 | eGFP Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1010 copies/ml control lentiviral particles ready for transduction, 25 µl | EF1α | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-EYFP-LV105-0200 | eYFP Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-EYFP-LV105-0205 | eYFP Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-EYFP-LV105-025 | eYFP Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1010 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-ECFP-LV105-0200 | eCFP Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-ECFP-LV105-0205 | eCFP Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-ECFP-LV105-025 | eCFP Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1010 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-MCHR-LV105-0200 | mCherry Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-MCHR-LV105-0205 | mCherry Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-MCHR-LV105-025 | mCherry Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1011 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-PLUM-LV105-0200 | PLUM Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-PLUM-LV105-0205 | PLUM Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-PLUM-LV105-025 | PLUM Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1010 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-FLUC-LV105-0200 | Firefly Luciferase Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-FLUC-LV105-0205 | Firefly Luciferase Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-FLUC-LV105-025 | Firefly Luciferase Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-RLUC-LV105-0200 | Renilla Luciferase Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-RLUC-LV105-0205 | Renilla Luciferase Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-RLUC-LV105-025 | Renilla Luciferase Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-MGLUC-LV105-0200 | Gaussia Luciferase Lentifect Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-MGLUC-LV105-0205 | Gaussia Luciferase Lentifect Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-MGLUC-LV105-025 | Gaussia Luciferase Lentifect Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-HLUC-LV201-0200 | Firefly luciferase + eGFP Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 107-108 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | eGFP/Puro |
LP-HLUC-LV201-0205 | Firefly luciferase + eGFP Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 107-108 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | eGFP/Puro |
LPP-HLUC-LV201-025 | Firefly luciferase +eGFP Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | >1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | eGFP/Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-HLUC-LV177-0200 | Firefly luciferase + IRES-mCherry Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | N/A |
LP-HLUC-LV177-0205 | Firefly luciferase + IRES-mCherry Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | N/A |
LPP-HLUC-LV177-025 | Firefly luciferase + IRES-mCherry Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | >1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | N/A |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-RLUC-LV177-0200 | Renilla luciferase + IRES-mCherry Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | N/A |
LP-RLUC-LV177-0205 | Renilla luciferase + IRES-mCherry Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | N/A |
LPP-RLUC-LV177-025 | Renilla luciferase + IRES-mCherry Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | >1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | N/A |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-NEG-LV105-0200 | Negative Control Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-NEG-LV105-0205 | Negative Control Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-NEG-LV105-025 | Negative Control Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1011 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
LP-NEG-LV151-0200 | Negative Control Lentifect Lentiviral Particles (200 µl x 1 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Neo |
LP-NEG-LV151-0205 | Negative Control Lentifect Lentiviral Particles (200 µl x 5 vial) | Crude | 109-1010 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Neo |
LPP-NEG-LV151-025 | Negative Control Lentifect Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1011 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Neo |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-CMYC-LV105-0200 | c-Myc Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-CMYC-LV105-0205 | c-Myc Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-CMYC-LV105-025 | c-Myc Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-KLF4-LV105-0200 | Klf4 Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 107-108 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-KLF4-LV105-0205 | Klf4 Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 107-108 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-KLF4-LV105-025 | Klf4 Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-OCT4-LV105-0200 | Oct4 Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-OCT4-LV105-0205 | Oct4 Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-OCT4-LV105-025 | Oct4 Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-SOX2-LV105-0200 | Sox2 Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 107-108 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-SOX2-LV105-0205 | Sox2 Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 107-108 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-SOX2-LV105-025 | Sox2 Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x108 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-Z8289-LV105-0200 | Nanog Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-Z8289-LV105-0205 | Nanog Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-Z8289-LV105-025 | Nanog Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1010 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-LIN28A-LV105-0200 | Lin28 Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-LIN28A-LV105-0205 | Lin28 Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-LIN28A-LV105-025 | Lin28 Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x1010 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-CRE-LVXM-0200 | CRE recombinase Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 107-108 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | eGFP Puromycin |
LP-CRE-LVXM-0205 | CRE recombinase Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 107-108 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | eGFP Puromycin |
LPP-CRE-LVXM-025 | CRE recombinase Purified Lentifect™ Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | eGFP Puromycin |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-FLP-LVXM-0200 | FLP recombinase Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | eGFP Puromycin |
LP-FLP-LVXM-0205 | FLP recombinase Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | eGFP Puromycin |
LPP-FLP-LVXM-025 | FLP recombinase Purified Lentifect™ Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | eGFP Puromycin |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-SV40T-LV105-0200 | SV40 large T antigen Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-SV40T-LV105-0205 | SV40 large T antigen Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-SV40T-LV105-025 | SV40 large T antigen Purified Lentifect™ Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
Catalog# | Product | Type | Description * | Promoter | Reporter/ Marker |
LP-TAT-LV105-0200 | HIV TAT Lentifect™ Lentiviral Particles (200 µl x 1 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 200 µl | CMV | Puro |
LP-TAT-LV105-0205 | HIV TAT Lentifect™ Lentiviral Particles (200 µl x 5 vial) | Crude | 108-109 copies/ml control lentiviral particles ready for transduction, 5 x 200 µl | CMV | Puro |
LPP-TAT-LV105-025 | HIV TAT Lentifect™ Purified Lentiviral Particles (25 µl x 1 vial) | Purified | > 1x109 copies/ml control lentiviral particles ready for transduction, 25 µl | CMV | Puro |
*Titer determined by quantitative PCR. | ![]() | |
数据表
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