Re-scan Confocal Microscopy (RCM)_详细资料

产品信息

货号： RCM 1.1

RCM features
RCM is an easy to use, sensitive, high resolution and affordable confocal imaging system:
- An ideal solution for small labs with limited budget, but demanding tasks, particularly when high sensitivity and resolution are desired from the imaging system,
- A confocal microscope that works as a camera, no need for an instruction manual
- RCM is extremely easy to use: no hardware control or software processing needed, and the images are always RAW.

RCM can be delivered from Confocal.nl as a total microscope system with a selection of microscopes ( Nikon, Olympus, Leica or Zeiss ), a selection of cameras ( Hamamatsu, PCO, Andor, Photometrics ) and laser solutions (Omicron, Toptica ).

In case you already have a microscope in the lab, RCM is an upgrade to an existing wide-field fluorescence system – RCM can easily be added to the existing wide-field fluorescence microscope system to improve its resolution.

The new RCM (1.1)

The new RCM (1.1) is based on RCM (1.0) and has all the same features. On top of these, the new RCM offers:
- Bypass mode
- Integration in SVI Huygens deconvolution software
- A scanning speed up to 4 frames per second
- Two times the field of view

RCM 1.1 is available in a VIS and NIR version.

Resolution: the standard resolution of the RCM is 170 nm at 488 nm wavelength. This is also called super-resolution. In contrast to other super-resolution systems, RCM offers the super-resolution of the live (raw) image without any processing. Deconvolution can be used to improve the RCM resolution even further: to 120 nm.

Frame rate: the frame rate of the RCM is 1 fps at 512 x 512, which makes the acquisition time of a 3 colour image about 3 seconds. The frame rate of RCM 1.1 can be increased to 4 fps at 512 x 512, which makes the acquisition time of a 3 colour image less than 1 second. With the new RCM 1.1 applications that require a bit faster imaging are now possible.

Field of view: RCM 1.0 has a Field of View (FOV) of 80 x 80 μm at 100x magnification, RCM 1.1 has the option to increase this FOV to 160 x 160 μm.
Standard mode – high resolution RCM image. FOV 80 x 80 micron

Increased FOV – standard confocal resolution. FOV 160 x 160 micron

Building blocks
RCM scanning unit is connected via standard C-mount adapters between the microscope and camera. The laser light is fed into the RCM unit via a single mode fibre connector (both standard connectors). The RCM comes in two versions: RCM-Visible and RCM-NIR. Visible is designed to operate as a 4-colour system in the visible range (400-700nm). NIR is designed to operate in the Near-Infrared (785nm excitation). The RCM comes with Bypass mode to allow for fast focusing in epi-fluorescence.

Microscopes
RCM can be mounted to any inverted microscope with a side-port. Motorised focussing unit (internal or external) is also needed in most applications. Currently, RCM has been tested with Nikon Ti & Ti2, Olympus IX, MiCube, Zeiss Axio Observer and Leica DMi6000 & DMi8.

Please note that:
- RCM only mounts correctly to the original microscope manufacturers C-mount
- A 100/0 beam splitter is mandatory. 50/50, 70/30 and 80/20 beam splitters do not work with RCM, as they are not laser safe.

The RCM fits on virtually any microscope. Here we see configurations with the 4 major microscope brands.

Objective lenses
In order to obtain the 170 nm resolution, the highest NA (>1.40) lenses available have to be used. In addition to that the camera needs to have a suitable pixel size (see below).

Cameras
Any microscope camera with a C-mount connector, external trigger, and similar specifications as the two first ones in the list below should work with the RCM. Please contact us, if you have a camera that you would like to test for the RCM compatibility.

Camera models tested to work with RCM:
- Hamamatsu Flash 4 V2 (LT) and V3,
- Andor Zyla, 888 and 897
- PCO Edge 4.2
- FLIR Chameleon 3 (Micromanager only, with custom driver)

A physical pixel size of 6.5 micron or lower is ideal for 60-100x objective lenses, as this results in sufficiently small sampling. At 100x objective magnification and 6.5 micron pixel the sampling is 43 nm at the sample plane.

Beamsplitter
- Hamamatsu W-View Gemini A12801-01
- Cairn Multisplit V2, four-way emission splitter

Software
RCM drivers are available for LabView (free of charge), Micro-Manager / ImageJ (Fiji) (free of charge), and Nikon NIS Elements (at cost).

Lasers
Laser combiners: Omicron Lighthub, Toptica CLE, Coherent Galaxy, Nikon Lu-N4, Oxxius and Vortran. The RCM laser coupling is compatible with single mode fibers with FC/APC connector and NA 0.11. Using other fiber connectors will degrade image quality.

Wavelengths: For RCM-Visible lasers with a wavelength from 405 upwards are suitable. RCM is designed as a 4 colour system. We offer a flexible laser solution that can be equipped with 1 to 4 lasers. Laser lines can be upgraded at later stage as well.

As the RCM is sensitive, we only need low power lasers (max 25 mW). The advantage of the low power lasers is the significantly longer lifetime and lower costs.

Other products

In case you have a microscope, camera, laser combiner or software that is not on the list, and you would like to use this in combination with RCM, please let us know!

Also, in case you are a manufacturer of a microscope, camera, laser combiner or software, and you would like to have your product in this list, please contact us for a test.

If any questions please contact Amy for 13651715049 (wechat) or 355887203@qq.com

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