pHCMC04 Catalog No. PVT11309
Packing 2ug
pHCMC04 Information
Promoter: xylA promoter
Prokaryotic resistance: ampicillin Amp
Screening markers: chloramphenicol Chl
Cloned strain: Escherichia coli DH5 alpha
Culture conditions: 37 degrees centigrade
Expression host: Bacillus subtilis
Inducement: xylose
pHCMC04 Description
PHCMC04 is a Bacillus subtilis xylose inducible expression plasmid.
pHCMC04 Sequence
LOCUS Exported 8089 bp ds-DNA circular SYN 06-JAN-2018
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..8089
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(440..1028)
/direction=LEFT
/label=ori
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1199..2059)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2060..2164)
/gene="bla"
/label=AmpR promoter
CDS complement(2343..2993)
/codon_start=1
/label=Chl
/translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
CDS complement(3497..4621)
/codon_start=1
/label=xylR
/translation="MNQKLILDEILKNSPVSRATLSEITGLNKSTVSSQVNTLLEKDFI
FEIGAGQSRGGRRPVMLVFNKNAGYSIGIDIGVDYLNGILTDLEGNIILEKTSDLSSSS
ASEVKEILFALIHGFVTHMPESPYGLVGIGICVPGLVDRHQQIIFMPNLNWNIKDLQFL
IESEFNVPVFVENEANAGAYGEKVFGMTKNYENIVYISINIGIGTGLVINNELYKGVQG
FSGEMGHMTIDFNGPKCSCGNRGCWELYASEKALLASLSKEEKNISRKEIVERANKNDV
EMLNALQNFGFYIGIGLTNILNTFDIEAVILRNHIIESHPIVLNTIKNEVSSRVHSHLD
NKCELLPSSLGKNAPALGAVSIVIDSFLSVTPIS"
misc_feature 4729..4882
/label=xylA promoter
misc_feature 4805..4829
/label=xylO
misc_feature 4898..4997
/label=MCS
ORIGIN
1 tcgaggcctg caggcatgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
61 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
121 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
241 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
301 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
361 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
421 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
481 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
541 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
601 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
661 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
721 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
781 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
841 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
901 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
961 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
1021 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
1081 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
1141 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
1201 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
1261 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
1321 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
1381 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
1441 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
1501 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
1561 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
1621 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
1681 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
1741 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
1801 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
1861 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
1921 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
1981 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
2041 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
2101 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
2161 cgcgcacatt tccccgaaaa gtgccacctg actgccgggc ctcttgcggg atcggcgagg
2221 ctagttaccc ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg
2281 tacctattaa tgtatcgttt tagaaaaccg actgtaaaaa gtacagtcgg cattatctca
2341 tattataaaa gccagtcatt aggcctatct gacaattcct gaatagagtt cataaacaat
2401 cctgcatgat aaccatcaca aacagaatga tgtacctgta aagatagcgg taaatatatt
2461 gaattacctt tattaatgaa ttttcctgct gtaataatgg gtagaaggta attactatta
2521 ttattgatat ttaagttaaa cccagtaaat gaagtccatg gaataataga aagagaaaaa
2581 gcattttcag gtataggtgt tttgggaaac aatttccccg aaccattata tttctctaca
2641 tcagaaaggt ataaatcata aaactctttg aagtcattct ttacaggagt ccaaatacca
2701 gagaatgttt tagatacacc atcaaaaatt gtataaagtg gctctaactt atcccaataa
2761 cctaactctc cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt
2821 gtcactaaga aaataaatgc agggtaaaat ttatatcctt cttgttttat gtttcggtat
2881 aaaacactaa tatcaatttc tgtggttata ctaaaagtcg tttgttggtt caaataatga
2941 ttaaatatct cttttctctt ccaattgtct aaatcaattt tattaaagtt catttgatat
3001 gcctcctaaa tttttatcta aagtgaattt aggaggctta cttgtctgct ttcttcatta
3061 gaatcaatcc ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat
3121 cccactttat ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaagac
3181 cacattaaaa aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc
3241 ttttctttct tatcttgata ataagggtaa ctattgccga tcgtccattc cgacagcatc
3301 gccagtcact atggcgtgct gctagcgcca ttcgccattc aggctgcgca actgttggga
温馨提示：不可用于临床ZL。
pHCMC04/ pHCMC04质粒/ pHCMC04 Plasmid
Omega质粒提取,Plasmid Maxi Kit(20)说明书
D6904 omega质粒中提 Plasmid Midi Kit
D6943 omega质粒提取 plasmid mini kit
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