lentiMPH v2
Catalog No. PVT10984
Packing 2ug
lentiMPH v2 Information
Function Mammal Editing plasmids
Prokaryotic resistance: ampicillin Amp
Screening markers: hygromycin Hyg, bleomycin Zeo
Cloned strain: Escherichia coli Stbl3
Culture conditions: 37 degrees centigrade
lentiMPH v2 Desciprion
LentiMPH V2 is a mammalian Cas9 expression plasmid.
lentiMPH v2 Sequence
LOCUS Exported 11739 bp ds-DNA circular SYN 04-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..11739
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 488..613
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
LTR 660..840
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
promoter complement(858..1056)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
enhancer 1058..1437
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1703..1807
/gene="bla"
/label=AmpR promoter
CDS 1808..2668
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2839..3427
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
polyA_signal 3878..3999
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS complement(4129..4503)
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=Zeo
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
promoter complement(4522..4569)
/label=EM7 promoter
/note="synthetic bacterial promoter "
promoter complement(4617..4946)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4631..4766
/label=SV40 ori
/note="SV40 origin of replication"
rep_origin complement(4960..5388)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
polyA_signal 5434..5658
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
LTR 5690..5870
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
promoter 6475..7653
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
regulatory 7660..7669
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 7669..8055
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
VKAMQGLLKDGNPIPSAIAANSGIY"
CDS 8110..8130
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 8146..8688
/codon_start=1
/product="C-terminal portion of the p65 subunit of mouse
NF-kappa-B"
/label=p65
/translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
FQQLLNQGVSMSHSTAE <
温馨提示：不可用于临床ZL。
lentiMPH v2/ lentiMPH v2质粒/ lentiMPH v2 Plasmid
Omega酵母质粒,Yeast Plasmid Kit (200)现货
金黄色葡萄球菌CRISPR/CAS9基因编辑质粒pCasSA (Plasmid #98211)
