pEYFP- N1/ pEYFP- N1质粒/ pEYFP- N1 Plasmid

pEYFP-N1

Search name

pEYFP-N1,Plasmid pEYFP-N1,pEYFP-N1 vector

 

 

pEYFP-N1 Information

Promoter: CMV

Replicon: pUC ori, F1 ori

Terminator: SV40 poly (A) signal

Plasmid classification: mammalian cells, fluorescent protein reporter vectors

Plasmid size: 4733bp

Prokaryotic resistance: Kan

Selection marker: Neo

Clonal strain: DH5 alpha

Culture conditions: 37

Expression host: lactation cells

Induction mode: no need to induce, transient expression.

5'sequencing primers: pEGFP-C-5:CATGGTCCTGCTGGAGTTCGTG

Primers for 3'sequencing: primers designed based on sequences

 

pEYFP-N1 Description

pEYFP-N1 encodes an enhanced yellow-green variant of the Aequorea victoria green fluorescent protein (GFP). The EYFP gene contains four amino acid substitutions previously published as GFP-10C (1). The fluorescence excitation maximum of EYFP is 513 nm, and the emission spectrum has a peak at 527 nm (in the yellow-green region). When excited at 513 nm, the Em of EYFP is 36,500 cm–1M–1 and the fluorescence quantum yield is 0.63 (1), resulting in a bright fluorescent signal. The fluorescence level observed from EYFP is roughly equivalent to that from EGFP.

 

pEYFP-N1 Multiple cloning site

 

 

pEYFP-N1 Sequence