pX459M 双靶点CRISPR 敲除系统 核心质粒 Puro

测序及更多信息：https://pan.baidu.com/s/1Jos_RCGzLCWp9dsV_hvvoQ 密码：6l46

采购地址：淘宝店铺地址

搭配过渡质粒pHH-hU6-A-gRNA(和B只是酶切位点不同)

或者

搭配过渡质粒pHH-hU6-B-gRNA(和A只是酶切位点不同) 

1        GAGGGCCTAT TTCCCATGAT TCCTTCATAT TTGCATATAC GATACAAGGC TGTTAGAGAG
61       ATAATTGGAA TTAATTTGAC TGTAAACACA AAGATATTAG TACAAAATAC GTGACGTAGA
121      AAGTAATAAT TTCTTGGGTA GTTTGCAGTT TTAAAATTAT GTTTTAAAAT GGACTATCAT
181      ATGCTTACCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA
241      CGAAACACCG GGTCTTCGAG AAGACCTGTT TTAGAGCTAG AAATAGCAAG TTAAAATAAG
301      GCTAGTCCGT TATCAACTTG AAAAAGTGGC ACCGAGTCGG TGCTTTTTTG TTTTAGAGCT
361      AGAAATAGCA AGTTAAAATA AGGCTAGTCC GTTTTTAGCG CGTGCGCCAA TTCTGCAGAC
421      AAATGGCTCT AGAGTCGACA CTAGTCTCGA GATCGATAAG CTTCCTAGAG GTACCCGTTA
481      CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT
541      CAATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA
601      CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA
661      ATGACGGTAA ATGGCCCGCC TGGCATTGTG CCCAGTACAT GACCTTATGG GACTTTCCTA
721      CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTCGAGGTG AGCCCCACGT
781      TCTGCTTCAC TCTCCCCATC TCCCCCCCCT CCCCACCCCC AATTTTGTAT TTATTTATTT
841      TTTAATTATT TTGTGCAGCG ATGGGGGCGG GGGGGGGGGG GGGGCGCGCG CCAGGCGGGG
901      CGGGGCGGGG CGAGGGGCGG GGCGGGGCGA GGCGGAGAGG TGCGGCGGCA GCCAATCAGA
961      GCGGCGCGCT CCGAAAGTTT CCTTTTATGG CGAGGCGGCG GCGGCGGCGG CCCTATAAAA
1021     AGCGAAGCGC GCGGCGGGCG GGAGTCGCTG CGACGCTGCC TTCGCCCCGT GCCCCGCTCC
1081     GCCGCCGCCT CGCGCCGCCC GCCCCGGCTC TGACTGACCG CGTTACTCCC ACAGGTGAGC
1141     GGGCGGGACG GCCCTTCTCC TCCGGGCTGT AATTAGCTGA GCAAGAGGTA AGGGTTTAAG
1201     GGATGGTTGG TTGGTGGGGT ATTAATGTTT AATTACCTGG AGCACCTGCC TGAAATCACT
1261     TTTTTTCAGG TTGGACCGGT GCCACCATGG ACTATAAGGA CCACGACGGA GACTACAAGG
1321     ATCATGATAT TGATTACAAA GACGATGACG ATAAGATGGC CCCAAAGAAG AAGCGGAAGG
1381     TCGGTATCCA CGGAGTCCCA GCAGCCGACA AGAAGTACAG CATCGGCCTG GACATCGGCA
1441     CCAACTCTGT GGGCTGGGCC GTGATCACCG ACGAGTACAA GGTGCCCAGC AAGAAATTCA
1501     AGGTGCTGGG CAACACCGAC CGGCACAGCA TCAAGAAGAA CCTGATCGGA GCCCTGCTGT
1561     TCGACAGCGG CGAAACAGCC GAGGCCACCC GGCTGAAGAG AACCGCCAGA AGAAGATACA
1621     CCAGACGGAA GAACCGGATC TGCTATCTGC AAGAGATCTT CAGCAACGAG ATGGCCAAGG
1681     TGGACGACAG CTTCTTCCAC AGACTGGAAG AGTCCTTCCT GGTGGAAGAG GATAAGAAGC
1741     ACGAGCGGCA CCCCATCTTC GGCAACATCG TGGACGAGGT GGCCTACCAC GAGAAGTACC
1801     CCACCATCTA CCACCTGAGA AAGAAACTGG TGGACAGCAC CGACAAGGCC GACCTGCGGC
1861     TGATCTATCT GGCCCTGGCC CACATGATCA AGTTCCGGGG CCACTTCCTG ATCGAGGGCG
1921     ACCTGAACCC CGACAACAGC GACGTGGACA AGCTGTTCAT CCAGCTGGTG CAGACCTACA
1981     ACCAGCTGTT CGAGGAAAAC CCCATCAACG CCAGCGGCGT GGACGCCAAG GCCATCCTGT
2041     CTGCCAGACT GAGCAAGAGC AGACGGCTGG AAAATCTGAT CGCCCAGCTG CCCGGCGAGA
2101     AGAAGAATGG CCTGTTCGGA AACCTGATTG CCCTGAGCCT GGGCCTGACC CCCAACTTCA
2161     AGAGCAACTT CGACCTGGCC GAGGATGCCA AACTGCAGCT GAGCAAGGAC ACCTACGACG
2221     ACGACCTGGA CAACCTGCTG GCCCAGATCG GCGACCAGTA CGCCGACCTG TTTCTGGCCG
2281     CCAAGAACCT GTCCGACGCC ATCCTGCTGA GCGACATCCT GAGAGTGAAC ACCGAGATCA
2341     CCAAGGCCCC CCTGAGCGCC TCTATGATCA AGAGATACGA CGAGCACCAC CAGGACCTGA
2401     CCCTGCTGAA AGCTCTCGTG CGGCAGCAGC TGCCTGAGAA GTACAAAGAG ATTTTCTTCG
2461     ACCAGAGCAA GAACGGCTAC GCCGGCTACA TTGACGGCGG AGCCAGCCAG GAAGAGTTCT
2521     ACAAGTTCAT CAAGCCCATC CTGGAAAAGA TGGACGGCAC CGAGGAACTG CTCGTGAAGC
2581     TGAACAGAGA GGACCTGCTG CGGAAGCAGC GGACCTTCGA CAACGGCAGC ATCCCCCACC
2641     AGATCCACCT GGGAGAGCTG CACGCCATTC TGCGGCGGCA GGAAGATTTT TACCCATTCC
2701     TGAAGGACAA CCGGGAAAAG ATCGAGAAGA TCCTGACCTT CCGCATCCCC TACTACGTGG
2761     GCCCTCTGGC CAGGGGAAAC AGCAGATTCG CCTGGATGAC CAGAAAGAGC GAGGAAACCA
2821     TCACCCCCTG GAACTTCGAG GAAGTGGTGG ACAAGGGCGC TTCCGCCCAG AGCTTCATCG
2881     AGCGGATGAC CAACTTCGAT AAGAACCTGC CCAACGAGAA GGTGCTGCCC AAGCACAGCC
2941     TGCTGTACGA GTACTTCACC GTGTATAACG AGCTGACCAA AGTGAAATAC GTGACCGAGG
3001     GAATGAGAAA GCCCGCCTTC CTGAGCGGCG AGCAGAAAAA GGCCATCGTG GACCTGCTGT
3061     TCAAGACCAA CCGGAAAGTG ACCGTGAAGC AGCTGAAAGA GGACTACTTC AAGAAAATCG
3121     AGTGCTTCGA CTCCGTGGAA ATCTCCGGCG TGGAAGATCG GTTCAACGCC TCCCTGGGCA
3181     CATACCACGA TCTGCTGAAA ATTATCAAGG ACAAGGACTT CCTGGACAAT GAGGAAAACG
3241     AGGACATTCT GGAAGATATC GTGCTGACCC TGACACTGTT TGAGGACAGA GAGATGATCG
3301     AGGAACGGCT GAAAACCTAT GCCCACCTGT TCGACGACAA AGTGATGAAG CAGCTGAAGC
3361     GGCGGAGATA CACCGGCTGG GGCAGGCTGA GCCGGAAGCT GATCAACGGC ATCCGGGACA
3421     AGCAGTCCGG CAAGACAATC CTGGATTTCC TGAAGTCCGA CGGCTTCGCC AACAGAAACT
3481     TCATGCAGCT GATCCACGAC GACAGCCTGA CCTTTAAAGA GGACATCCAG AAAGCCCAGG
3541     TGTCCGGCCA GGGCGATAGC CTGCACGAGC ACATTGCCAA TCTGGCCGGC AGCCCCGCCA
3601     TTAAGAAGGG CATCCTGCAG ACAGTGAAGG TGGTGGACGA GCTCGTGAAA GTGATGGGCC
3661     GGCACAAGCC CGAGAACATC GTGATCGAAA TGGCCAGAGA GAACCAGACC ACCCAGAAGG
3721     GACAGAAGAA CAGCCGCGAG AGAATGAAGC GGATCGAAGA GGGCATCAAA GAGCTGGGCA
3781     GCCAGATCCT GAAAGAACAC CCCGTGGAAA ACACCCAGCT GCAGAACGAG AAGCTGTACC
3841     TGTACTACCT GCAGAATGGG CGGGATATGT ACGTGGACCA GGAACTGGAC ATCAACCGGC
3901     TGTCCGACTA CGATGTGGAC CATATCGTGC CTCAGAGCTT TCTGAAGGAC GACTCCATCG
3961     ACAACAAGGT GCTGACCAGA AGCGACAAGA ACCGGGGCAA GAGCGACAAC GTGCCCTCCG
4021     AAGAGGTCGT GAAGAAGATG AAGAACTACT GGCGGCAGCT GCTGAACGCC AAGCTGATTA
4081     CCCAGAGAAA GTTCGACAAT CTGACCAAGG CCGAGAGAGG CGGCCTGAGC GAACTGGATA
4141     AGGCCGGCTT CATCAAGAGA CAGCTGGTGG AAACCCGGCA GATCACAAAG CACGTGGCAC
4201     AGATCCTGGA CTCCCGGATG AACACTAAGT ACGACGAGAA TGACAAGCTG ATCCGGGAAG
4261     TGAAAGTGAT CACCCTGAAG TCCAAGCTGG TGTCCGATTT CCGGAAGGAT TTCCAGTTTT
4321     ACAAAGTGCG CGAGATCAAC AACTACCACC ACGCCCACGA CGCCTACCTG AACGCCGTCG
4381     TGGGAACCGC CCTGATCAAA AAGTACCCTA AGCTGGAAAG CGAGTTCGTG TACGGCGACT
4441     ACAAGGTGTA CGACGTGCGG AAGATGATCG CCAAGAGCGA GCAGGAAATC GGCAAGGCTA
4501     CCGCCAAGTA CTTCTTCTAC AGCAACATCA TGAACTTTTT CAAGACCGAG ATTACCCTGG
4561     CCAACGGCGA GATCCGGAAG CGGCCTCTGA TCGAGACAAA CGGCGAAACC GGGGAGATCG
4621     TGTGGGATAA GGGCCGGGAT TTTGCCACCG TGCGGAAAGT GCTGAGCATG CCCCAAGTGA
4681     ATATCGTGAA AAAGACCGAG GTGCAGACAG GCGGCTTCAG CAAAGAGTCT ATCCTGCCCA
4741     AGAGGAACAG CGATAAGCTG ATCGCCAGAA AGAAGGACTG GGACCCTAAG AAGTACGGCG
4801     GCTTCGACAG CCCCACCGTG GCCTATTCTG TGCTGGTGGT GGCCAAAGTG GAAAAGGGCA
4861     AGTCCAAGAA ACTGAAGAGT GTGAAAGAGC TGCTGGGGAT CACCATCATG GAAAGAAGCA
4921     GCTTCGAGAA GAATCCCATC GACTTTCTGG AAGCCAAGGG CTACAAAGAA GTGAAAAAGG
4981     ACCTGATCAT CAAGCTGCCT AAGTACTCCC TGTTCGAGCT GGAAAACGGC CGGAAGAGAA
5041     TGCTGGCCTC TGCCGGCGAA CTGCAGAAGG GAAACGAACT GGCCCTGCCC TCCAAATATG
5101     TGAACTTCCT GTACCTGGCC AGCCACTATG AGAAGCTGAA GGGCTCCCCC GAGGATAATG
5161     AGCAGAAACA GCTGTTTGTG GAACAGCACA AGCACTACCT GGACGAGATC ATCGAGCAGA
5221     TCAGCGAGTT CTCCAAGAGA GTGATCCTGG CCGACGCTAA TCTGGACAAA GTGCTGTCCG
5281     CCTACAACAA GCACCGGGAT AAGCCCATCA GAGAGCAGGC CGAGAATATC ATCCACCTGT
5341     TTACCCTGAC CAATCTGGGA GCCCCTGCCG CCTTCAAGTA CTTTGACACC ACCATCGACC
5401     GGAAGAGGTA CACCAGCACC AAAGAGGTGC TGGACGCCAC CCTGATCCAC CAGAGCATCA
5461     CCGGCCTGTA CGAGACACGG ATCGACCTGT CTCAGCTGGG AGGCGACAAA AGGCCGGCGG
5521     CCACGAAAAA GGCCGGCCAG GCAAAAAAGA AAAAGGAATT CGGCAGTGGA GAGGGCAGAG
5581     GAAGTCTGCT AACATGCGGT GACGTCGAGG AGAATCCTGG CCCAATGACC GAGTACAAGC
5641     CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG
5701     CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC GGACCGCCAC ATCGAGCGGG
5761     TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG
5821     TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG
5881     CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC
5941     AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG
6001     CCACCGTCGG AGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC
6061     CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC
6121     GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG
6181     AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGAGAATTC TAACTAGAGC
6241     TCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC
6301     CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA
6361     AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA
6421     CAGCAAGGGG GAGGATTGGG AAGAGAATAG CAGGCATGCT GGGGAGCGGC CGCAGGAACC
6481     CCTAGTGATG GAGTTGGCCA CTCCCTCTCT GCGCGCTCGC TCGCTCACTG AGGCCGGGCG
6541     ACCAAAGGTC GCCCGACGCC CGGGCTTTGC CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG
6601     CAGCTGCCTG CAGGGGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC
6661     ACACCGCATA CGTCAAAGCA ACCATAGTAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG
6721     GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT
6781     TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT
6841     CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT
6901     GATTTGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG
6961     ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC
7021     CCTATCTCGG GCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA
7081     AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGTTTACA
7141     ATTTTATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA
7201     CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC
7261     AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG
7321     AAACGCGCGA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA
7381     ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT
7441     TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA
7501     ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT
7561     ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA
7621     GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC
7681     AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT
7741     AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT
7801     CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT
7861     CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC
7921     ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG
7981     CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC
8041     ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA
8101     CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG
8161     GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT
8221     GATAAATCTG GAGCCGGTGA GCGTGGAAGC CGCGGTATCA TTGCAGCACT GGGGCCAGAT
8281     GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA
8341     CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC
8401     CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC
8461     TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC
8521     CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG
8581     CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG
8641     GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA
8701     AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG
8761     CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG
8821     TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA
8881     ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC
8941     CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT
9001     CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC
9061     TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA
9121     TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC
9181     CTGGCCTTTT GCTGGCCTTT TGCTCACATG T