WI-38[WI38]细胞系—人胚肺成纤维细胞系全国减价

WI-38[WI38]细胞系—人胚肺成纤维细胞系全国减价 货号：GD-C16819186

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系—全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞系—其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
N-(2-Hydroxybenzoyl)pyrrolidine, 97%
(R)-(+)-2-Diphenylphosphino-2'-Methoxy-1,1'-binaphthyl
3-Ethoxy-2-fluorophenylboronic acid
cis-2,4,5-TriMethoxy-1-propenylbenzene
1,3-Difluoro-5-iodobenzene
DIBENZOFURAN-4-BORONIC ACID
SulfaMic acid
4-Amino-2,3,5,6-tetrafluoropyridine
Acryloyl Chloride (stabilized with Phenothiazine)
3,5-Difluoro-4-hydroxybenzaldehyde 97%
4-Methylbenzeneboronic acid, pinacol ester
4-Benzyloxybenzaldehyde
2-Buthylimidazole
4-HEPTYLANILINE
2-AMino-2-thioxoethyl pivalate
PalMitoyl chloride
Diallyldiphenylsilane
p-Xylylene Dithiocyanate
Uric acid, 99+%
SodiuM Monofluorophosphate, USP
gaMMa-Decanolactone
clofibrate
Piperidine hydrochloride
2,5-dibromo-p-xylene
3-Hydroxytetrahydrofuran
1-Hydroxy-1-cyclopropanecarboxylic acid
Undecan-4-olide
2,3-DiMethyl-5-ethylpyrazine
SodiuM tetraborate decahydrate, 99+%
Vinyl Methacrylate, 98%, stab. with 200ppM 4-Methoxyphenol
5,15-Diphenylporphyrin
MethyltrioxorheniuM(VII)
4-(4,4,5,5,6,6,7,7,8,8,9,9,10,10,11,11,11-Heptadecafluoroundecyloxy) benzaldehyde
Pentyl chloroforMate
0
2-BroMo-3-fluorobenzonitrile
2-(4-Bromophenyl)ethyl Alcohol
(4-Pyrid-3-ylphenyl)methanol, 97%
-MSH, human
2-Bromo-1-iodo-4-methoxybenzene
5-Norbornene-2-carbonitrile
2-Cyano-5-methylpyridine
(S)-3-(Boc-amino)-4-phenylbutyric acid
Colchicine
Mercaptoacetic acid
Methyl 3-nitrosalicylate
Ethyl 2-AMinobenzothiazole-6-carboxylate
5-AMino-1-(3-broMophenyl)-4-cyano-3-Methylpyrazole
(2-Methylphenyl)Methanesulfonyl chloride, 97%
PERYLENE
2-Phenylethyl ForMate
mepivacaine hydrochloride
Allyl methyl sulfide
3-Bromoanisole
2-Amino-4-picoline
4-Hydroxybenzyl cyanide
DiMethyl fuMarate
6-CHLOROTHIOCHROMAN-4-ONE
BARIUM NIOBIUM OXIDE
Ethyl 1-benzylpiperidine-4-carboxylate 96%
DL-(4-Chlorophenyl)glycine
5-Amino-3-methyl-4-isoxazolecarbonitrile
Lithium phenoxide, 0.6M solution in THF, AcroSeal
3-Fluorophenol
TRIETHYL 3-PHOSPHONOPROPIONATE
3-AMINO-2,4,5-TRIFLUOROBENZOIC ACID
3,3-Dimethyl-D(-)-cysteine
2-BroMobenzonitrile
1-Benzyl-4-cyano-4-hydroxypiperidine Hydrochloride
3-ChloroMethylphenylboronic acid
Brilliant cresyl Blue, pure, high purity biological stain
(?)-Mevalonolactone, 97%
N,N'-DiisopropylethylenediaMine
N,N',N''-Triacetylchitotriose
1-Iodo-2-fluoroethane
(4'-bromobiphenyl-4-yl)boronic acid
N-fmoc-ethanolamine
Potassium hydroxide
CA15-3 ELISA Kit
Mouse TAF1 (TATA Box Binding Protein Associated Factor 1) ELISA Kit
Human SOAT2 ELISA Kit
Human PANK2 ELISA Kit
IL1β ELISA Kit
Rat alpha-L-fucosidase,AFU ELISA Kit
Chicken Nuclear factor-kappa B,NF-κB ELISA Kit
Human MCP-4/CCL13 ELISA Kit
Human HbH ELISA Kit
FUM ELISA Kit
Letheen Broth Base ,Modified
Dey/Engley Neutralizing Agar Base
IndiuM(III) ethoxide, 99.9% (Metals basis)
Sodium tetrafluoroborate
5,7-Dodecadiyne-1,12-diol, 96%
2-Fluoro-5-(trifluoroMethyl)cinnaMic acid, 97+%
clofibrate
1-methyl-3-pyrrolidinol
3-(4-hydroxyphenyl)-1-propanol
Curcumin

Cyclohexanecarboxaldehyde
Silver Carbonate
n-Propyl forMate, 97%
2-Methyl-3,5-dinitrobenzoic acid, 98%
PotassiuM hydrogen phosphate, dried, 98+%
4-Benzyloxyindole-3-carboxaldehyde
Cinchonine Monohydrochloride hydrate
POTASSIUM CARBONATE, SESQUIHYDRATE
LANTHANUM OXALATE
2-Acetyl-3,5(6)-diMethylpyrazine, Mixture of isoMers
3-MethylaMino-1,2-propanediol
Methyl perfluorododecanoate
4-[(4-Methoxybenzylidene)amino]cinnamic Acid
2-Bromo-3-fluorophenylacetic acid
4-Ethynylbenzoic acid methyl ester