SW1353细胞系*人骨肉瘤细胞系优惠价热卖 货号:GD-C15419273
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系*全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞系*其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
(S)-(+)-1-Methoxy-2-propylaMine, ChiPros 98+%, ee 96+%
N-NitrosodiphenylaMine
(S)-3-AMinopyrrolidine
Zein
2-Methyl-2-propyl-1,3-propanediol
BOC-D-THR-OH
1-NAPHTHALENEMETHYLAMINE
6-bromo-3-pyridazinol(SALTDATA: FREE)
Cyclohexylamine, 99%
1,2-Diamino-2-methylpropane
2,5-Bis(4-biphenylyl)thiophene
3-CHLORO-2,6-DIBROMO-4-METHYLANILINE
DIETHYL HYDRAZODICARBOXYLATE
4-Methylthiophene-2-boronic acid
L-alpha-Cyclohexylglycine
3-Chloro-2-fluorobenzenesulfonyl chloride
Di-tert-butylphenylphosphine
2-BroMo-6-fluorobenzaldehyde
(R)-N-BOC-Prolinal, 97+%
Sulfathiazole, Powder, USP
3-BroMobenzotrifluoride
1-(2-hydroxy-3-methylphenyl)ethanone
4,4-diethoxybutylamine
Oxacillin sodium anhydrous
Benzenediazonium, 3-bromo-, tetrafluoroborate(1-)
1(P-METHOXYPHENYL)-3-BUTYLAMINE
TetrabutylaMMoniuM fluoride trihydrate
AntiMony(III) ethoxide, 99.9% (Metals basis)
3,3,3-Trifluoropropionic acid, 98%
N,N-DiMethyl-n-octylaMine, 95%
2,4-Dichlorophenoxyacetic acid
N-Boc-tyraMine
2-Fluoro-3,5-dichloropyridine
O-(4-Chlorobenzoyl)hydroquinine
4-(N-EthylaMinocarbonyl)phenylboronic acid
4-BROMO-2-ETHYLACETANILIDE
N-propylcyclopropanamine(SALTDATA: FREE)
2-Chloro-5-[3,4-dihydro-1(2H)-quinolinylsulfonyl]-aniline
(2-amino-4,6-dimethyl-5-pyrimidinyl)acetic acid(SALTDATA: FREE)
N-(3-amino-4-methylphenyl)acetamide(SALTDATA: FREE)
6-Benzyloxyindole
2-Methylpropanedioic acid
4,6-DINITRO-O-CRESOL SODIUM SALT
3-Acetyl-1-propanol
1,3-Thiazol-2-ylMethylaMine
Bis(Methyldiphenylphosphine)palladiuM(II) Dichloride
3-Chloro-2-Methoxyphenylboronic acid
Boiling stones, for analytical work
2,5-Dichloro-4-(trifluoroMethyl)pyridine, 97+%
8-Azahypoxanthine
1-(2,4-DiMethylphenyl)
Perfluoro(2-methyl-3-pentanone)
oxo(1H-pyrrolo[2,3-b]pyridin-3-yl)acetic acid
5-EthylPyridine-2,3-Dicarboxylic Acid Diethyl Ester
4-Chloro-2-iodophenol
1-Chloro-N,N,2-trimethylpropenylamine
1,3-Dibenzyloxybenzene
2,5-Difluorophenol
Ethyl 3,4-dichlorophenylacetate, 97+%
alpha,alpha-Dimethyl-beta-methylsuccinimide, 99+%
3-(4-Pyridyl)acrylic acid
2,4-Dimethyl-6-nitrophenol
Zirconocene Dichloride
ELAIDIC ACID
PHOSPHOMOLYBDIC ACID HYDRATE
DL-2-AMINOBUTYRIC ACID
Disulfur dichloride
(3-Chlorophenyl)Methanol
1-Butyl-1-MethylpyrrolidiniuM BroMide
2-Methoxy-1-naphthaldehyde
N-Cyclopropyl-2-nitro-4-trifluoroMethylaniline
1,2-DibroMo-1,2-diphenylethane, 96%
Nitrogen
2-(tert-ButoxycarbonylaMino)ethyl BroMide
3-IODOPYRIDINE-4-CARBONITRILE
Pectolinarin
Hydroxy Propyl Methyl Cellulose
3,3-Dimethylglutaric anhydride
Porcine AR (Amphiregulin) ELISA Kit
Mouse Qa lymphocyte antigen 2 region,Qa-2 ELISA Kit
Human PCDH22 ELISA Kit
DPP-4 ELISA Kit
Chicken factor B,BF ELISA Kit
Rat Aβ40 (Amyloid Beta 40) ELISA Kit
Chicken heparan sulfate,HS ELISA Kit
Human EMILIN 1 (Elastin Microfibril Interface Located Protein 1) ELISA Kit
Human serum amyloid A3,SAA3 ELISA Kit
IgA ELISA Kit
Korser’s Citrate Broth
DYS Yersinaia Agar
Ethyl acetopyruvate, 98%
Lithium fluoride
COPPER TPP
2-FLUORO-3-(TRIFLUOROMETHYL)BENZYL BROMIDE
1-(2-hydroxy-3-methylphenyl)ethanone
2,4-Dimethylthiophenol
Tracid Brilliant Red 10b
3-nitropropionic acid
3-BENZOYLPROPIONIC ACID
DICHLOFLUANID
4-Benzyloxyindole
(R)-(-)-3-Fluoropyrrolidine hydrochloride, 97%
(R)-3-(4-Fluorophenyl)-3-(FMoc-aMino)propionic acid, 95%
Phosphonitrilic chloride triMer
PotassiuM tetraphenylborate
BISMUTH MOLYBDENUM OXIDE
3-[2-(PERFLUOROHEXYL)ETHOXY]-1,2-EPOXYPROPANE
Boc-(S)-α-(4-fluorobenzyl)-Pro-OH
2,4,5-TRIBROMOTOLUENE
1-Formyl-4-piperidinecarboxylic Acid
3,4,5-Trimethoxyphenylboronic acid, 97%
4-Chloro-1-naphthol
5-Hydroxyquinoline ,98%