HOS细胞系*人骨肉瘤细胞系优惠价热卖 货号:GD-C15418929
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系*全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
常见细胞培养中一些问题总结:1)培养基中血清的比例多少合适?血清是细胞培养上一个极为重要的营养来源,所以血清的含量多少会对细胞的生长会产生极大的影响。血清的含量一般为5%-15%,不要低于5%或高过20%,因为含量过低会导致细胞营养不足,过高则会破坏渗透压平衡。一般建议血清含量为10%,可以适量加减;2)何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上的更换时间,按时更换培养基即可。还可以参照指示剂的颜色变化进行更换;3)购买的复苏细胞系*为何会有脱落?因为运输的过程中不可避免的会有震荡,收到后可以离心收集;4)购买的细胞冷冻管经解冻后,为何会发生细胞数目太少之情形?研究人员在冷冻细胞之培养时出现细胞数目太少,大都是因为离心过程操作上的失误,造成细胞的物理性损伤,以及细胞流失。建议细胞解冻后不要立刻离心,应待细胞生长隔夜后再更换培养基即可;5)购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,常见原因可归纳为:培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后,加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80°C太久;6)收到的冷冻管瓶身破裂,瓶盖有裂纹,或瓶盖脱落之原因?冷冻管瓶盖裂纹,或瓶身破裂,可能是因为操作者夹取冷冻管时用力不当,造成冷冻管裂损,建议使用止血钳小心夹取。另冷冻管瓶盖松动或松脱,乃因热胀冷缩之物理现象,冷冻管有可能因此而造成细胞污染,故冷冻管于放入和取出液氮桶时,均应立刻将冷冻管再一次扭紧。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
2-Fluoro-4-(Methylthio)benzeneboronic acid, 98%
4-n-Propylphenylacetylene
Boc-Ala-ONp
6-BroMo-2-diethylaMinopyridine
Chloropentafluorobenzene
1-(2-BROMOETHOXY)-4-CHLOROBENZENE
AntiMony
2-(Morpholin-4-yl)acetamide
1,2-Benzanthraquinone
Ethyl 2-Amylacetoacetate
Eugenol Acetate
4-Choro-2(3H)-benzothiazolone
2-Chlorobenzonitrile
n-Butyllithium
4-Bromo-2-methoxyphenol
2-AMino-3-broMopyridine
2,3-DiMethylanthraquinone
3,5-DibroMo-2-hydroxy-4-(trifluoroMethyl)pyridine
TitaniuM(IV) chloride, 1M solution in toluene, AcroSeal
Pentylenetetrazole
Nalpha-[(9H-Fluoren-9-ylMethoxy)carbonyl]-D-lysine Hydrochloride
3-Amino-4,4,4-trifluorobutyric acid
1,2,3,4,5,6,7,8,8-nonachloro-2,3,3a,4,7,7a-hexahydro-1H-4,7-methanoindene
2-Phenylbutyronitrile
4-ethoxyphenylurea
Sodium dichloroacetate
2-QUINOXALINECARBONYL CHLORIDE
1-BroMo-3-Chloro-2-Methylpropane
2-Chloro-4-fluorothiophenol, 97%
9-AMinofluorene hydrochloride, 99%
Glyoxylic acid anhydrous
3-Methylvaleric acid
N-PENTADECANOIC ACID ETHYL ESTER
Rubeanic Acid , 99%
Diethyl (2-oxobutyl)phosphonate
N-BUTYLIDENEPHTHALIDE
1-Methyl-4-(4-nitrophenyl)
3-Phenylphthalide
N-(3-phenyl-2-propyn-1-yl)cyclopropanamine(SALTDATA: HCl)
Octanoic acid, 99%
(S)-3-Chloro-1-phenyl-1-propanol
DIPHENYLACETALDEHYDE
3-Hydroxy-4,5-dimethoxybenzoic acid
PotassiuM broMide;
Tetrahydropyran-2-Methanol
(MethoxycarbonylMethyl)triphenylphosphoniuM broMide
Pentafluoro-L-phenylalanine
1,3-Dihydroxynaphthalene, 99+%
4-BroMo-1-Methyl-1H-pyrazole-3-carbonitrile, 97%
Aspartame
3,4-Dihydroxybenzoic Acid
methylaminodimethylchlorosilane
N-benzhydrylsilyl-N-methyl-methanamine
protostemonine
4-methylumbelliferyl sulfate potassium salt
2-Furaldehyde diethyl acetal
2-Phenylazo-4-Methylphenol , 98%
N-Cyclohexyl 3-boronobenzenesulfonaMide
(2S,4S)-4,5-Dihydro-4-isopropyl-2-(p-tolyl)-1,2λ4,3-oxathiazole 2-oxide
Bis(1,5-cyclooctadiene)rhodium(I) trifluoromethane sulphonate 98+%
5-(4-Chlorophenyl)-2-(trifluoromethyl)furan-3-carboxylic acid
2,4-Nonadecadiynoic Acid
L-Proline β-naphthylamide hydrochloride ,95%
4-PROPIONYLBIPHENYL
2-[(1S,2S)-1-Ethyl-2-(phenylmethoxy)propyl]hydrazinecarboxaldehyde
2-N-BUTYL-5-ETHYLTHIOPHENE
Benzyl glycinate p-toluenesulfonate
4-Fluoro-3-(trifluoroMethyl)phenacyl broMide
1,3-Butadiene
Vinyl Laurate (stabilized with MEHQ)
2-BroMo-4-thiocyanatoaniline
D(+)-Malic acid, 98%
3'-Hydroxyacetophenone
Valeronitrile
4-Methylbenzoylacetonitrile
6-bromo-2-methyl-3-nitropyridine
5-Bromo-6-chloro-3-indolyl phosphate p-toluidine salt
6-Methoxy-1,3-benzothiazole
NN-T4 ELISA Kit
Mouse β-Glu (Beta Glucosidase) ELISA Kit
Human Lpa ELISA Kit
Human FUR ELISA Kit
IgG ELISA Kit
L-selectin ELISA Kit
PGFM ELISA Kit
E2 ELISA Kit
Human Motilin,MTL ELISA Kit
ACO-2 ELISA Kit
Phytone Yeast Extract Agar
Pseudomonas Isolation Agar
Ethyl Isonicotinate
GRAM'S IODINE
5-AMino-2-Methylpyridine, 97%
2-Hexene, cis + trans, tech. 85%
3-Amino-4,4,4-trifluorobutyric acid
ETHYL 5-NORBORNENE-2-CARBOXYLATE (MIXTURE OF ENDO AND EXO)
beta-cyclodextrin
malonic acid
4-N-DODECYLRESORCINOL
Pimelic acid
1,2,3,4-Tetrahydronaphthalene
2,4-Difluoro-3-Methoxybenzonitrile, 97%
MethylMagnesiuM chloride, 3M in THF
TiMosaponin A-Ⅲ
N,N-DicyclohexylMethylaMine
(3-METHYL-2-THIENYL)METHYLAMINE
Hexafluoro-2,2-diphenylpropane , 98%
2-(TrifluoroMethyl)benzenesulfonaMide
α-Methyl-L-phenylalanine
Methyl 3-amino-5-(tert-butyl)thiophene-2-carboxylate, 97%
Methyl perfluoro-3,6-dioxaheptanoic acid
Nalpha-Carbobenzoxy-DL-asparagine
Perfluoro(2-methyl-3-pentanone) 98%