U-2 OS细胞系*人骨肉瘤细胞系优惠价热卖 货号:GD-C15419119
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞系*全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息,我们拥有丰富的细胞系培养经验,能提供细胞培养全方位的技术支持,让您实验再无烦扰!公司由在国外工作和学习多年的留学人员创办,由国内ZG科学院上海生物化学与细胞生物学研究所,复旦大学,同济大学等多名工作在线的细胞培养专家,提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念,以世界的技术支撑阵容,提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美,提供了重要的基石;公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品,立足国内,面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位,为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内,在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室,以便更好的服务国内广大医院、大学、制药企业等,你们的每一份关心和支持,都是我们前进的动力!
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞系*其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
Benzotrichloride, 98%
3,4-Dichloroaniline
1-CYCLOPROPYLETHANOL
2-Chloro-1-fluoro-4-Methylsulfonylbenzene
N-Methyl 3-nitrobenzenesulfonaMide
Glycerol trivalerate
METHYL 6-HYDROXY-2-NAPHTHOATE
3-Bromo-N-methyl-5-nitrobenzamide 98%
Isosorbide dimethyl ether, 99%
H-89, Dihydrochloride Salt >99%
2-(benzyloxy)-5-bromobenzaldehyde(SALTDATA: FREE)
Reinecke salt
Methyl dimethoxyacetate
Isobutaneboronic acid
2-BroMovaleric acid
2-Methyl hydrogen pyridine-2,5-dicarboxylate
2'-Deoxy-5-Methylcytidine
Undecanophenone
Triphenylphosphinecopper(I) hydride hexaMer, 97%
S-(+)-Mandelic Acid
2,3-DibroMo-3-phenylpropionic Acid
syn-benzaldehyde oxime
L-Homocysteine
isopropylmagnesium chloride solution
2-Chloro-6-fluorocinnamic acid
6-Aminobenzothiazole
FMOC-L-Valine
2-BroMo-3-Fluoro-4-Picoline
2-(2,6-DiMethylphenylcarbaMoyl)benzoic acid, 97%
4-Iodotoluene, 98%
3-AMino-1-propanesulfonic acid sodiuM salt
Nitrofurazone
4-(1-Piperidinyl)benzaldehyde
PhenyltriMethylaMMoniuM broMide
5-AMINO-4-BROMO-3-METHYLPYRAZOLE HYDROBROMIDE
2-Fluoro-5-nitrobenzonitrile
6-Chloro-2,4(1H,3H)-pyrimidinedione
Ethyl 2-bromo-3-oxo-3-phenylpropanoate
3-Bromohexane (stabilized with Copper chip)
FITC I hydrochloride
(R)-1-(4-CHLOROPHENYL)ETHYLAMINE
2,3-DIMETHYLFURAN
2,4,6-Trifluorobenzaldehyde
Phenanthrenequinone
1-Chloro-4-nitrobenzene
SodiuM pyrophosphate
Triphenylborane - Triphenylphosphine CoMplex
2-BroMo-3-Methylpyridine, 95%
Cyclopentadienyltungsten tricarbonyl diMer, 97%
Phosphorus oxybromide
1-ChloroMethylpyrene
1-Fluoro-2,4,6-Trimethylpyridinium triflate
(3R)-4-(benzyloxy)-3-[(tert-butoxycarbonyl)amino]-4-oxobutanoic acid (non-preferred name)
methyl 4-hydroxy-3-methylbenzoate
Uracil 5-carboxylic acid Hydrate
2-Amino-1,3-propanediol
cis-2-AMino-2-Methylcyclohexanecarboxylic acid hydrochloride
L-Histidine
N-Ethyl-D-glucaMine
1-Eicosene
Cyclopiazonic acid, Penicillium cyclopium
4-Acetyl-1H-pyrrole-2-carboxylic acid
N-methyl-2-phenoxyethanamine(SALTDATA: FREE)
Fmoc-(S)-3-Amino-3-phenylpropionic acid
Imperatorin
2-Bromo-2-methylpropionyl bromide
Ethyl vanillin
2,3-Difluoroaniline
1-AdaMantaneethanol
3-(3,4-Dichlorophenyl)benzoic acid
4-AMino-4H-1,2,4-triazole, 99%
(2-AMinopyrid-4-yl)Methanol, 97%
Glycine methyl ester hydrochloride
Isopropyl Methacrylate (stabilized with MEHQ)
Hexadecyl dimethyl ethyl ammonium bromide
1-boc-
ALKYLATED DIPHENYLAMINES
5-Chlorothiophene-2-carbonyl chloride
OMT ELISA Kit
ZBP1 ELISA Kit
APOM ELISA Kit
Human SPIN2 ELISA Kit
Chicken C3 (Complement Component 3) ELISA Kit
TLR-9 ELISA Kit
Chicken infectious coryza,ic ELISA Kit
Human colicin ELISA Kit
Human Angiopoietin 2,ANG-2 ELISA Kit
BK ELISA Kit
MS-H Medium
NBB Broth Base,Modified
Isopropylboronic acid pinacol ester, 98%
1alpha,25-Dihydroxycholecalciferol
5-Chloro-2-fluorobenzonitrile, 97+%
2-Fluoro-6-Methoxypyridine, 97%
syn-benzaldehyde oxime
Sodium bismuthate
Polychloroprene
2-Fluoro-4-chlorotoluene
Methyl 2-chloropropionate
CIS-1-BROMO-1-PROPENE
3-Nitrobenzeneboronic Acid
(Mesitylene)tungsten tricarbonyl, 98%
1-(o-Tolyl), 97%
3'-AMinoacetophenone, 97%
Iron acetylacetonate
2,2-DIPHENYLPROPIONIC ACID
2,3-DIFLUORO-4-METHYLBENZYL BROMIDE
Ethyl 2-acetylcaprylate
2,4,6-TRI-TERT-BUTYLNITROBENZENE
COMANIC ACID
L-Arginine methyl ester dihydrochloride, 98%
Undecyl Ether
Methyl 3-(cyclopropylamino)-2-(2,4-dichloro-5-fluorobenzoyl)acrylate