CCK-81人结肠癌腺癌细胞系液体培养基 货号：GD-CP130020
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞接收操作说明：请仔细阅读本操作说明及相应的细胞说明书。本公司细胞发货有四种形式：T25培养瓶(一般是贴壁细胞使用，悬浮细胞也可以)、离心管(悬浮细胞使用多，当然，有些公司也会用这种离心管形式发贴壁细胞，但是，长期经验来说，贴壁细胞容易发生形态变化，估计是细胞培养空间狭小，血清不足导致(Z主要是怕运输延误导致这些情况发生)，个人不建议用离心管形式发贴壁细胞)及干冰(冻存细胞用，主要是冬天天气冷，温度低于4℃的地方，都要考虑冻存细胞)。T25培养瓶：是用T25细胞培养瓶直接发货的形式。收到细胞后，拆开包装T25培养瓶的自封袋，不拆封口膜，表面喷75%酒精消毒后显微镜观察细胞状态(有条件可以拍下此时细胞照片)。将细胞放入37度培养箱平衡两小时以上，再处理细胞。首先将T25中培养基取出装好备用，如细胞密度高于80%，可以直接消化传代，相反则加入5-6ml培养基放回培养箱继续培养即可。离心管：是用15ml离心管发货的形式，只用于悬浮细胞发货。收到细胞后消毒处理及将细胞放入37度培养箱平衡两小时以上，再处理细胞。首先将T25中培养基取出装好备用(如果实验室没有T25培养瓶，可以暂时T75培养瓶，加入10-15ml培养基，建议10ml培养基，也可以使用T175培养瓶，加入50-60ml培养基，培养瓶越大，需要的培养基越多。当然如果刚接收到细胞密度不够的话，Z好不用大瓶子，先用小瓶子养起来再换大的，不然会长得很慢，严重的，会导致细胞死亡等危险)，平衡完成后，离心(1000rpm，3min)收集细胞，弃上清，用新的培养基重悬细胞并接种至培养瓶或皿中，放回培养箱继续培养。干冰：是用本公司冻存液冻存细胞后，直接用干冰将冻存的细胞冷冻发货的形式。收到细胞后按照细胞复苏的步骤操作即可。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
1H-Benzotriazole, 99%
Chlorhexidine
(4R,5R)-1,3-DIMETHYL-4,5-DIPHENYL-2-[(S)-1-BENZYL-2-HYDROXYETHYLIMINO]IMIDAZOLIDINE
2,5-DIBROMOTHIAZOLE
ETHYL ACETOACETATE PROPYLENE GLYCOL KETAL
TRI-N-PROPYLTIN ACETATE
N-(2-Chloroethyl) 3-boronobenzaMide
4-(Tetrahydropyran-4-yloxy)benzoic acid , 97%
2-Bromo-1-(3,4-dihydro-2H-1,5-benzodioxepin-7-yl)ethan-1-one, 97%
3-Acetamido-3-deoxy-D-glucose ,98%
Didecyl Disulfide
2-[3-Methyl-4-(2,2,2-trifluoroethoxy)-2-pyridinyl]methylthio-1H-benzimidazole
1-PENTYN-3-OL
Pamidronate disodium salt
3,4-Difluorobenzaldehyde
1-(Methylsulfonyl)-4-nitrobenzene
(R)-1,2-DiaMinopropane Dihydrochloride
N-t-Butyl 4-(4-broMopyrazol-1-yl)benzenesulfonaMide
TetraMethylaMMoniuM hydroxide, 25 wt% in Methanol
N-Lauroylsarcosine SodiuM Salt, Ultrapure
2-AMino-4-Methylbenzonitrile
Copper(I) cyanide
Acetyl-Thiazolidine-4-Carboxylic acid
1-Pentadecyne
2,2',4,4'-Tetrehydroxybenzophenone
5-IODO-2-METHOXYANILINE
1,3-DIHYDROXYACETONE DIMER
Tert-Butyl 1H-pyrazole-1-carboxylate
Methyl 2-hydroxy-5-nitrobenzoate, 98%
3-BroMopropionic acid, 97%
4-Morpholinoaniline
4-(TrifluoroMethylthio)benzoic acid
(S)-(?)-2,3-Dibenzyloxy-1-propanol
SINIGRIN
2-(TrifluoroMethyl)biphenyl-3-carboxylic acid
2-ISOPROPENYLTHIAZOLE
3-(chloromethyl)-5-methyl-1,2,4-oxadiazole(SALTDATA: FREE)
Potassium dichromate, 4 wt% solution in water, pure
2-(4-Chlorobutoxy)tetrahydropyran
(1-Amino-1,3-dimethylbutyl)phosphonic acid, hydrate, 97%
N-(CHLOROACETYL)-3-(TRIFLUOROMETHYL)ANILINE
4-Isopropoxyaniline
4-Isopropoxyethoxymethylphenol
LithiuM Carbonate;Carbolith;CarbolithiuM;Lithonate
1,3-Benzodioxol-5-ylacetyl chloride
KaeMpferol
2,5-DiMethyl-1-phenylpyrrole
1-(4-Chlorobenzyl), 98%
2-[4-(ChloroMethyl)piperidino]pyriMidine, 97%
2-Methoxypyrazine
Methyl Myristate [Standard Material for GC]
2-amino-4-ethoxypyridine
(3aR,4S,5R,6aS)-4-(tert-Butyldimethylsilyloxy)methyl-5-hydroxy-hexahydro-2H-cyclopenta[b]furan-2-one
Ethyl 3-cyclopropyl-3-oxopropanoate
Trimethyl orthovalerate
1-BROMO-2,3,6-TRIFLUOROBENZENE
1,1'-Ferrocenedicarboxylic Acid
Gly-His hydrochloride hydrate
2-(4-Nitrophenoxy)naphthalene , 98%
3-(Isopropylcarbamoyl)-5-nitrobenzeneboronic acid 98%
3-Aminobenzenethiol
(R)-1-Cbz-beta-Prolinol
Lysozyme Chloride from Egg white
PENTAFLUOROPROPIONAMIDE
Perfluorotriethylamine
3,5-Bis(trifluoromethyl)phenylacetic acid
PATENT BLUE V
Tris(2-aMinoethyl)aMine
1,2-DiMethyl-3-propyliMidazoliuM Iodide
cis-3-Hexen-1-yl 2-Nonynoate
H-Leu-Phe-NH2 HCl
2-{[(9H-Fluoren-9-ylMethoxy)carbonyl]aMino}-3-Methylbutanoic acid, 97%
CHROMIUM(III) ACETYLACETONATE
2-Methoxythiophene
4-tert-Butylcyclohexanol, mixture of isomers
9,9-Bis[4-(Aminophenoxy)Phenyl]Fluorene (Baofl)
N-(2',6'-dimethylphenl)-2-Piperidine carboxamide
Ethyl 3-aminocrotonate
VEGFR1 ELISA Kit
Mouse OIT3 (Oncoprotein Induced Transcript 3) ELISA Kit
A-FABP ELISA Kit
Human voltage-gated calcium channel,VGCC ELISA Kit
GC ELISA Kit
GREM1 ELISA Kit
Monkey ApoB100 (Apolipoprotein B100) ELISA Kit
Human FGF3 ELISA Kit
Human TP ELISA Kit
FE ELISA Kit
Oxacillin Resistance Screening Agar Base
MSE Agar
Indole-6-Carboxylic Acid Methyl Ester
MERCURIC NITRATE, MONOHYDRATE
5-AMinoisoquinoline
2-Methyl-3-(Methylthio)furan
Copper(I) cyanide
lipoprotein lipase F. chromobact. visc.
Prometryne
2-Mercaptobenzothiazole
2-Chloroanthracene
POLYOXYETHYLENE(30) DOCOSYL ETHER
Butyl levulinate
EuropiuM(II) fluoride, anhydrous, 99.9% (Metals basis)
Isovaleric acid, 98%
M-DiMethoxy benzene
2-Chloroethyl ether
LITHIUM TRIFLUOROACETATE
Triethylsilane
Xylenol orange tetrasodiuM salt
Ethyl furaneol
(-)-Menthol
Triphenylacetoxytin
4-Hydroxy-1-naphthaldehyde
6-Methoxy-1,2,3,4-tetrahydronaphthalene
