NCI-H28[H28] Cell:人恶性间皮瘤复苏传代细胞系 货号:GD-C6520431
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤:【瘤细胞悬液接种】1)无菌选取生长良好(有光泽,淡红色)瘤组织或对数生长期培养瘤细胞;2)在PBS中将瘤组织剪碎后用匀浆器研磨,经80~100目筛网过滤成细胞悬液;3)培养细胞应用PBS洗两遍;4)计数并调整细胞浓度至107~108/ml;5)常规消毒后,于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml/部位,>106细胞)。初次接种成功率低,细胞数尽可能多一些;6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期,以后随着传代潜伏期逐渐缩短,固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位,引起腹水,腹水中含有瘤细胞,将这种腹水反复传代,即可成为腹水瘤。初次传代时,腹水常呈血性(含大量红细胞),反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤,进行细胞计数;2)消毒动物,左下腹穿刺接种106腹水瘤细胞;3)接种腹水瘤细胞后约7~12d,待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部,用9号针头抽取腹水,也可行腹部解剖后,用滴管吸取。每只小鼠可抽3~5ml;4)抽取的腹水经3000rpm离心15min,收集上清,分装冻存备用。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
3-BroMo-4-Methoxybenzoic acid, 98+%
4-BroMo-3,5-difluoroanisole
Propyl isocyanate
9,9-Bis(4-broMophenyl)fluorene
1,2-DibroMobenzene
2-(BENZYLOXY)-3-METHOXYBENZALDEHYDE
2-BroMotetradecane, 95%
5-amino-1-propyl-1H-imidazole-4-carboxamide(SALTDATA: FREE)
(S)-N-Boc-(2'-Chlorophenyl)glycine
1,2,4-Triaminobenzene Dihydrochloride
4-(Isobutyramido)benzeneboronic acid 98%
1,3-DIPHENOXYBENZENE
4-Bromo-1-fluoro-2-nitrobenzene
4-AMINO-TEMPO
1,2-CyclohexanedicarboxiMide
2-Acetoxyisobutyryl chloride
3,4'-Dihexyl-2,2'-bithiophene
3-BroMo-4-ethoxybenzaldehyde
Sudan Orange G, pure
SulfaMethizole
Isopropyl Acetoacetate
3-Nitrobenzamide
N-Benzylglycine ethyl ester hydrochloride
3-Acetyloxypregn-16-en-20-one
2,4-Pyridinedicarboxylic acid
9-HYDROXY-4-METHOXYACRIDINE
CARPROPAMID
Methyl Crotonate
SodiuM octanoate, 96%
2-Chloro-5-Methoxybenzoic acid, 98%
2-NP-AOZ
β-Gentiobiose
3-(Piperidine-1-carbonyl)phenylboronic acid, pinacol ester
O-Acetyl-N-carbobenzoxyhydroxylaMine
2-AMino-3-broMo-5-chloro-4-Methylpyridine
2-Acetoxyphenylboronic acid, pinacol ester
N-BOC-4-Chloroaniline, 98%
4-Benzyloxybromobenzene
1H,1H,2H,2H-Perfluorooctyl acrylate 95%
Disodium Citrate Sesquihydrate
Hexaethylcyclotrisiloxane
PHTHALIMIDOMALONIC ACID DIETHYL ESTER
3-Methylphthalic anhydride
1-PROPOXY-2-PROPANOL
Triphenylphosphine
L-Tyrosine ethyl ester hydrochloride
2,2,6,6-TetraMethyl-4-piperidyl Methacrylate
2-Furoic acid, 98%
AMMoniuM hexafluorosilicate, 99.999%, (trace Metal basis)
BOC-N-methyl-D-alanine
3,5-Dichlorobenzoic Acid
3-(4-BROMOBENZYL)-2-BUTYL-4-CHLORO-1H-IMIDAZOL-5-YLMETHANOL
2-chloroisonicotinamide
N-(3-Butenyl)phthalimide
3-chloro-6-methyl-dibenzo[c,f][1,2]thiazepin-11(6H)-one 5,5-dioxide
1,4-DICHLORO-5,6,7,8-TETRAHYDRO-5,8-ETHANOPHTHALAZINE
TRIETHYL 2-PHOSPHONOBUTYRATE
TRANS-2-OCTENOIC ACID
γ-(2-Naphthyl)-γ-butyrolactone
3-[(4-Methylpiperazin-1-yl)methyl]benzaldehyde, 95%
2-Butyl-4,4,5,5-tetramethyl-[1,3,2]dioxaborolane
1,5-Diiodopentane (stabilized with Copper chip)
2-Thiopheneglyoxal hydrate 95%
Acetonitrile
Flunixin meglumin
Dichloromonofluoromethane
TopiraMate
2-BroMoethyl ether
1-BroMotridecane
2-Methoxy-5-nitrobenzonitrile
6-Methoxyquinoline, 98%
4-Phenyltetrahydropyran-4-carboxylic acid, 97%
4-NitrodiazoaMinobenzene
p-Naphtholbenzein
phytosphingosine hydrochloride
2-(Boc-amino)-4-bromopyridine
2,4-Dichloro-5-fluorobenzaldehyde
Methyl-13C-amine hydrochloride
AQP1 ELISA Kit
RORC ELISA Kit
Human PCDHα1 ELISA Kit
Human DPP10 (Dipeptidyl Peptidase X) ELISA Kit
CD4 ELISA Kit
Rat amyloid beta peptide 1-42,Aβ1-42 ELISA Kit
GS-ANA ELISA Kit
Human ELA3A ELISA Kit
Human Serum amyloid A,SAA ELISA Kit
INHβA ELISA Kit
Urease Agar Base
Modified Phosphate Saline Buffer
Zinc(II) Acetylacetonate
LutetiuM(III) fluoride, 99.9% (Metals basis)
5,5',6,6'-Tetrahydroxy-3,3,3',3'-tetraMethyl-1,1'-spirobisindane, 97%
2-Fluoro-3-Methylpyridine, 97%
3-Nitrobenzamide
2',3',4'-Trichloroacetophenone
Aluminum oxide
(E)-2-Buten-1-ol
1,2-O-Isopropylidene-alpha-D-xylofuranose
PHENETHYLZINC BROMIDE
5-Fluoro-2-nitroanisole
3-BroMopropylboronic acid pinacol ester, 98%
3-Fluoro-4-Methoxyphenylacetonitrile, 99%
Ioxynil-octanoate
2′,3′-Dideoxyuridine
3,4-Dihydro-7-hydroxy-2(1H)-quinolinone
4-Propoxy-3-Methylphenylboronic acid
BISPHENOL A DIMETHACRYLATE
3-(DipropylcarbaMoyl)phenylboronic acid
tert-Butyl 4-(3-aminobenzyl)-1-carboxylate, 97%
2,6-Dimethyl-4-methoxybenzeneboronic acid
2,5-Difluoro-4-methoxybenzonitrile ,99%
Ethyl 3-(trifluoromethyl)benzoate