NCI-H2452[H2452] Cell:人间皮瘤复苏传代细胞系 货号:GD-C6519091
细胞来源:细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支)
产品规格:≥1*10(6)/ml
安全级别:1
物种来源:人源或鼠源等
细胞形态:上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因?研究人员在细胞培养时出现存活率不佳,原因比较复杂,常见原因可归纳为:培养基使用错误或培养基品质不佳;血清使用错误或血清的品质不佳;解冻过程错误;冷冻细胞解冻后,加以洗涤细胞和离心;悬浑细胞误认为死细胞;培养温度使用错误;细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来,其离心速率应为多少转速?欲回收动物细胞,其离心速率一般为300×g(约1OOOrpm),5-10分钟,转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2,其中r为离心机转轴ZX与离心套管底部内壁的距离;rpm为离心机每分钟的转数;RCF(relative centrifugal force)为相对离心力,以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性:贴壁/悬浮等
细胞检测:细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件:详见细胞说明书
传代方法:建议1:2-1:3传代;两天换液一次(具体详细询问公司细胞培养专家)
(S,S)-DIPAMP, 97%
HydroxylaMMoniuM chloride
PotassiuM triphenylborohydride solution
Bis(tetrabutylaMMoniuM) TetracyanodiphenoquinodiMethanide , 98%
DOTA
N-BOC 5-broMo-2,3-difluoroaniline
M-TOLUALDEHYDE 2,4-DINITROPHENYLHYDRAZONE
5-isocyanato-1,3-benzodioxole(SALTDATA: FREE)
D-Mannonic acid-1,4-lactone ,98%
Ethyl 3-trifluoromethylbenzoylacetate
Methyl 4-bromo-3-hydroxybenzoate 98%
4-Bromophenylboronic acid
3,4-Dihydroxybenzonitrile
4-Methylcyclohexyl amine
2-Ethylpyrazine
AluMiniuM oxide
DiMethyl Octadecanedioate
N-Isopropyl 4-(4-broMopyrazol-1-yl)benzenesulfonaMide
Tri-n-butylphosphine, 95%, AcroSeal
PyriMethaMine, USP
N,N,N',N'-Tetrakis(2-pyridylMethyl)ethylenediaMine
m-Tolylacetic acid
1-acetylguanidine
2-Nitro-4-cymene
4-Fluorophenyl sulfone
1,2,2,6,6-Pentamethyl-4-piperidinol
TERT-BUTYLDIMETHYL(2-PROPYNYLOXY)SILANE
BenzaMide
LanthanuM aluMinuM oxide substrate, 10x10x1MM, polished 2 sides, 100 orientation
Methyl 3-chloro-4-Methoxybenzoate, 98%
2-Methylindole, 98+%
1-Hexyl-3-MethyliMidazoliuM tetrafluoroborate
(S)-(6,6′-DiMethoxybiphenyl-2,2′-diyl)bis[bis(4-Methylphenyl)phosphine]
PIPEROIN
5-Chloro-2-fluorobenzaMide, 97+%
O-(2-TRIMETHYLSILYLETHYL)HYDROXYLAMINE HYDROCHLORIDE
7-(TRIFLUOROMETHYL)-4-QUINOLINOL
S-Trityl-3-mercaptopropionic acid
3-Nitro-5-(trifluoromethyl)benzoic acid 97%
6-Amino-3-methyluracil ,97%
Boric acid gel, particle size 0.1-0.4 mm activated and swollen, pure
1,10-Dibromodecane
3-Bromo-2,4,5-trifluorobenzoic acid
Chlorhexidine digluconate
2-Bromo-5-methylpyridine
1-BroMo-3-phenoxybenzene
2,3-DiMethylfuran
N-Cyclohexyl 3-broMobenzenesulfonaMide
TetraphenylantiMony broMide
Tributyl Phosphate
TetraMethylgerMane
turanose
methyl 5-chloro-2-hydroxy-3-nitrobenzoate
4-fluoro-2-methylpyridine
Dibenzofuran
2,3,4-TRICHLORONITROBENZENE
1-TRIMETHYLSILYL-1,2,4-TRIAZOLE
3-AMINOPHTHALIC ACID HYDROCHLORIDE
3-BroMo-5-fluoroanisole
Samarium(III) fluoride (99.9%-Sm) (REO)
4,4'-Diaminobenzanilide
Diethyl Allylmalonate
1,3,5-Tri-O-benzoyl-α-D-ribofuranose ,98%
ISOXAZOLE-5-CARBOXYLIC ACID
6-AMINO-1,3-DIPROPYLURACIL
Iodine monochloride
Donepezil Hydrochloride
1,8-Octanediol
14-Azido-3,6,9,12-tetraoxatetradecanol
4-(Benzyloxy)-3,5-dichlorophenylboronic acid
4-Methoxy-3-pyridineboronic acid hydrate, 97%
Borane-dicyclohexylphosphine coMplex, 95%
4'-Hydroxyacetophenone
2-ThiophenecarboxaMide
thymidine 5'-triphosphate
6-bromo-2-chloroquinazolin-4(1H)-one
Ramoplanin
6-methyl-2,3-pyridinedicarboxylic acid
PGA ELISA Kit
Mouse α2-Antiplasmin,α2-AP ELISA Kit
Human EGR1 ELISA Kit
Human Stem cell factor/mast cell growth factor,SCF/MGF ELISA Kit
TSH ELISA Kit
Tau ELISA Kit
malonyl CoA ELISA Kit
Human OTR (Oxytocin Receptor) ELISA Kit
Human VEGFR-1/Flt1 ELISA Kit
ACE ELISA Kit
Murashige & Skoog Medium
Anaerobe Basal Agar
IsobutylaMine, 99%
Glycine ethyl ester hydrochloride, 99%
5-Chloro-2-hydroxybenzaMide, 97%
2-Fluoro-N-(2,6-difluorophenyl)benzaMide, 97%
m-Tolylacetic acid
C.I. Reactive blue 39
Tween(R) 60
methyl 2-fluorobenzoate
NIOBIUM SILICIDE
3,5-Dichlorobenzoic acid
2-Acetylanthracene, 97%
1,2-Epoxydecane, 97%
1,2,4-Trichloro-5-iodobenzene, 98%
TriethylMethylaMMoniuM chloride
1-(4-IODOPHENYL)PYRROLE
5-(4-Chlorophenyl)-2-furonitrile
1-BUTANEPHOSPHONIC ACID
N-Alpha-t-Boc-D-aspartic beta-cyclohexyl ester
CeriuM(III) iodide, ultra dry, 99.9% (REO)
C-(2,3-Dihydro-benzo[1,4]dioxin-2-yl)-methylamine
4-Tetrahydro-1H-pyrrol-3-ylpyridine
3,4-Dihydro-2H-1,5-benzodioxepine-7-carbonyl chloride
Losartan Potassium