Human Adiponectin ELISA Kit (Sandwich ELISA) - LS-F24347 Type: Quantitative Sandwich ELISA Format: 96-Well Strip Plate Reactivity: Human Range: 62.5-4000 pg/ml Detection: Colorimetric - 450nm (TMB) Sample Types: Cell Culture Supernatants, Serum, Tissue Homogenates, PlasmaProduct DescriptionLS-F31510 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat Adiponectin in samples of Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates and Urine. It is based upon a Sandwich assay principle and can be used to detect levels of Adiponectin as low as 10 picograms per milliliter. About Adiponectin Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Q15848 NM_004797 NP_004788.1
Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates, Urine Format
96-Well Strip Plate Detection
Colorimetric - 450nm (TMB) Measurement
Quantitative Detection Range
156 - 10000 pg/ml Sensitivity
10 pg/ml Storage
Short term: 4°C; Long term: see manual. Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Kit Components
Coated 96-well Strip Plate
Adhesive Plate Sealers
Sample Diluent
HRP-Streptavidin Conjugate Diluent
Wash Buffer (20X)
TMB Substrate
Stop Solution
Biotinylated Detection Antibody (100X)
Biotinylated Detection Antibody Diluent
HRP-Streptavidin Conjugate (100X)
Standard (Lyophilized)
Restrictions
For research use only. Guarantee
This elisa kit carries the LSBio Guarantee. Top of PagePublications (0)Submit a Publication Top of PageCustomer Reviews (0)Submit a Review Top of PageImages