Human Adiponectin ELISA Kit (Sandwich ELISA) - LS-F24347 Type: Quantitative Sandwich ELISA Format: 96-Well Strip Plate Reactivity: Human Range: 62.5-4000 pg/ml Detection: Colorimetric - 450nm (TMB) Sample Types: Cell Culture Supernatants, Serum, Tissue Homogenates, PlasmaProduct DescriptionLS-F5126 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the detection of Rabbit Adiponectin in samples of Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of Adiponectin as low as 0.313 nanograms per millilter. About Adiponectin Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Q15848 NM_004797 NP_004788.1
Adiponectin (ADP) No significant cross-reactivity or interference between Adiponectin (ADP) and analogs was observed. Manual
Intended Sample Types
Plasma, Serum Format
96-Well Strip Plate Detection
Colorimetric - 450nm (TMB) Detection Range
0.313 - 20 ng/ml Sensitivity
0.313 ng/ml Assay Time
4.5 h Precision
Intra-Assay: CV<10% Inter-Assay: CV<12% Storage
Short term: 4°C; Long term: see manual. Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Kit Components
Coated 96-well Strip Plate
Adhesive Plate Sealers
Standard (Lyophilized)
Sample Diluent
Detection Reagent A (Green)
Detection Reagent B (Red)
Assay Diluent A
Assay Diluent B
TMB Substrate
Stop Solution
Wash Buffer (30x concentrate)
Restrictions
For research use only. Guarantee
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