Human Adiponectin ELISA Kit (Sandwich ELISA) - LS-F24347 Type: Quantitative Sandwich ELISA Format: 96-Well Strip Plate Reactivity: Human Range: 62.5-4000 pg/ml Detection: Colorimetric - 450nm (TMB) Sample Types: Cell Culture Supernatants, Serum, Tissue Homogenates, PlasmaProduct DescriptionLS-F24710 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse Adiponectin in samples of Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates and Urine. It is based upon a Sandwich assay principle and can be used to detect levels of Adiponectin as low as 10 picograms per milliliter. About Adiponectin Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Q15848 NM_004797 NP_004788.1
For the detection and quantification of endogenous levels of natural and/or recombinant Mouse Adiponectin proteins. Manual
Intended Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates, Urine Format
96-Well Strip Plate Detection
Colorimetric - 450nm (TMB) Measurement
Quantitative Detection Range
156 - 10000 pg/ml Sensitivity
10 pg/ml Storage
Short term: 4°C; Long term: see manual. Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Kit Components
Coated 96-well Strip Plate
Standard (Lyophilized)
Biotinylated Detection Antibody (100x)
Avidin-Biotin-HRP Complex (100x)
Sample Diluent
Biotinylated Detection Antibody Diluent
Avidin-Biotin-HRP Complex Diluent
Wash Buffer (20x)
TMB Substrate
Stop Solution
Adhesive Plate Sealers
Background
Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. Restrictions
For research use only. Guarantee
This elisa kit carries the LSBio Guarantee. Top of PagePublications (0)Submit a Publication Top of PageCustomer Reviews (0)Submit a Review Top of PageImages