Caspase-1, human recombinant proteinase

产品说明Caspase-1，半胱氨酸蛋白酶，在大肠杆菌中表达。
该产品包含在以下化合物库中:

质量控制

• 纯度 =

化学性质

CAS号
别名
分子式	分子量
溶解性	储存条件

 实验操作

细胞实验[2]：
细胞系
溶解方法
反应时间
应用
动物实验[3]：
动物模型
剂量
注意事项
References:

产品描述Caspase-1 (also know as Interleukin-1β-Converting Enzyme) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme, which is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the mechanism of regulation of caspase-1 activation is complex and poorly understood. The recombinant active human caspase-1 was expressed in E. coli. The active caspase-1 preferentially cleaves caspase-1 substrates (YVAD-AFC or YVAD-pNA) and is routinely tested for its ability to enzymatically cleave Ac-YVAD-pNA or Ac-YVAD-AFC. The recombinant human Caspase-1 has an N-term His-tag and corresponds to amino acids 120-404 of Caspase-1.Active caspase-1 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.