Cy5.5 NHS ester (non-sulfonated)

• 纯度 = 98.00%

Cyanine5.5 NHS ester是用于标记含有伯胺的多肽，蛋白和寡核苷酸的反应性染料。 Cy5.5-NHS ester是一种近红外荧光染料，能够溶解在DMF和DMSO中，激发光和发射光分别在675 nm和693 nm左右[1]。该试剂不仅用于标记含有伯基的分子，而且还用于标记质粒DNA [2]。在生物分子标记中，该标记试剂的水溶性较低，先使用有机溶剂溶解该分子对于GX标记是必需的。首先，Cyanine染料应该溶解在有机溶剂中，然后加入包含生物分子的合适缓冲水溶液中。

通过皮下移植肿瘤小鼠的光学成像，在0.5nmol Cy5.5-NHS注射后30min至24h，皮下U87MG肿瘤可与周围背景组织明显区分开来。 Cy5.5-NHS的肿瘤吸收在注射后30分钟达到值，随着时间的推移缓慢洗出[3]。

References:

[1].  Ju Hee Ryu, Jin Hee Na, Ho Kyung Ko, et al. Non-invasive optical imaging of cathepsin B with activatable fluorogenic nanoprobes in various metastatic models. Biomaterials, 2014, 35: 2302-2311.
[2].  Laura-Lee Farrell, Joel Pepin, Cezary Kucharski, et al. A comparison of the effectiveness of cationic polymers poly-L-lysine (PLL) and polyethylenimine (PEI) for non-viral delivery of plasmid DNA to bone marrow stromal cells (BMSC). European Journal of Pharmaceutics and Biopharmaceutics, 2007, 65: 388–397.
[3].  Zhen Cheng, Jelena Levi, Zhengming Xiong, et al. Near-Infrared Fluorescent Deoxyglucose Analog for Tumor Optical Imaging in Cell Culture and in Living Mice. Bioconjug Chem., 2006, 17(3):662-669.