There’s no need to worry about your antibody or streptavidin peroxidase conjugates becoming inactive over time when you use new Guardian Peroxidase Stabilizer/Diluent (S/D), which preserves peroxidase activity from 1:100 to 1:100,000 dilution (Figures 1 and 3). Guardian S/D Formulation can be used to create stock solutions (1:1,000) or ready-to-use assay reagents (1:100,000) that can be stored at 4°C for 12 months or at room temperature for six months. No more wasting money with HRP dilutions you throw away the same day.
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