泛-甲基化（ε-N）赖氨酸抗体 Methylated (ε-N) Lysine Antibody_详细资料

产品信息

抗体英文名： Methylated (ε-N) Lysine Antibody 浓度： 250ug/ml 应用范围： ELISA,WB,IHC 是否单克隆：多克隆保存条件： -20 形态：液体规格： 100ug

Product Description

The affinity purified rabbit polyclonal anti-methylated lysine antibody was developed using a technique unique to ImmuneChem. The methylated lysine antibodies are affinity purified using N-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The antibodies could be utilized for detection, quantization and isolation of proteins with methylated lysine residues.

Western blot analysis of the methylated histone from melanoma cells with anti-methylated lysine (anti-MeK, ICP0501). Histones were isolated with cold 0.1N HCl extraction followed by the method.

A: blot with 0.5 ug/mL of anti-MeK (ICP0501) in TBSt and blocked with synthetic methylated BSA 100 ug/mL).

B: blot with 0.5 ug.mL of anti-MeK (ICP0501) in TBSt.

Species

Rabbit

Formulation

250 µg/mL in Tris-phosphate buffer, 50% Glycerol.

Immunogen

Methylated KLH conjugates

Purification

The antibody was immunoaffinity purified with epsilon N-methyl lysine on agarose

Specificity

The antibody recognizes proteins or peptides methylated on the lysine residues (mono, di-methylysine). There is no cross-reaction with acetylated proteins. The cross-reactivity with tri-methyl-lysine has not been tested.

Applications

ELISA, WB, Immunohistochemistry.

Scientific Description

MeK is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome C, chromosomal proteins, histones, non-histones as well as neural storage body proteins. It has been suggested that methylation of lysine plays an important role in gene silencing.^[1-3]

Storage & Stability

Store product at –20 ^oC. Three years from date of shipping.

References

1. Im, H. et al. J. Biol. Chem. 2003. 278: 18346-18352.
2. Ng, H. H. et al. J. Biol. Chem.2002. 277: 34655-34657.
3. Zegerman, P. et al. J. Biol. Chem. 2002. 277: 11621-11624.

相关产品：
Di-methyl (ε-N) Lysine Antibody ICP0701
Di-methyl (ε-N) Lysine Antibody, HRP   ICP0702
Dimethyl Asymmetric Arginine Antibody   ICP0810
Methyl Lysine Antibody,HRP   ICP0502
Methyl Mono Arginine Antibody   ICP0801
Methyl BSA Competitive Inhibitor   ICP0504
Methyl Lysine Antibody,Biotin   ICP0503
Methylated (ε-N) Lysine Antibody   ICP0501
Methylated Lysine Antibody, FITC   ICP0505
NG,NG-dimethyl Arginine Antibody (anti-dMeR), HRP ICP0812
NG-monomethyl Arginine Antibody (anti-mMeR), HRP   ICP0802
Tri-methyl BSA Competitive Inhibitor   ICP0604
Trimethyl Lysine Antibody   ICP0601
Trimethyl Lysine Antibody,Biotin   ICP0605
Trimethyl Lysine Antibody,FITC   ICP0603
Trimethyl Lysine Antibody,HRP   ICP0602
Methyl Lysine Antibody, Agarose   ICP0588
Trimethyl Lysine Antibody, Agarose   ICP0606

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Product Description	The affinity purified rabbit polyclonal anti-methylated lysine antibody was developed using a technique unique to ImmuneChem. The methylated lysine antibodies are affinity purified using N-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The antibodies could be utilized for detection, quantization and isolation of proteins with methylated lysine residues.	Western blot analysis of the methylated histone from melanoma cells with anti-methylated lysine (anti-MeK, ICP0501). Histones were isolated with cold 0.1N HCl extraction followed by the method. A: blot with 0.5 ug/mL of anti-MeK (ICP0501) in TBSt and blocked with synthetic methylated BSA 100 ug/mL). B: blot with 0.5 ug.mL of anti-MeK (ICP0501) in TBSt.
Species	Rabbit
Formulation	250 µg/mL in Tris-phosphate buffer, 50% Glycerol.
Immunogen	Methylated KLH conjugates
Purification	The antibody was immunoaffinity purified with epsilon N-methyl lysine on agarose
Specificity	The antibody recognizes proteins or peptides methylated on the lysine residues (mono, di-methylysine). There is no cross-reaction with acetylated proteins. The cross-reactivity with tri-methyl-lysine has not been tested.
Applications	ELISA, WB, Immunohistochemistry.
Scientific Description	MeK is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome C, chromosomal proteins, histones, non-histones as well as neural storage body proteins. It has been suggested that methylation of lysine plays an important role in gene silencing.^[1-3]
Storage & Stability	Store product at –20 ^oC. Three years from date of shipping.
References	1. Im, H. et al. J. Biol. Chem. 2003. 278: 18346-18352. 2. Ng, H. H. et al. J. Biol. Chem.2002. 277: 34655-34657. 3. Zegerman, P. et al. J. Biol. Chem. 2002. 277: 11621-11624.